PMID: 3762877Jul 1, 1986Paper

A specific radioimmunoassay for [Leu] and [Met]enkephalin applied to a HPLC separation of human CSF

Neuropeptides
S J CapperJ S Morley

Abstract

Highly specific antisera have been raised to [Leu]enkephalin and applied in a radioimmunoassay that is sufficiently sensitive and precise for the measurement of enkephalin pentapeptides in body fluids. Extraction of CSF by Sep-pak cartridges, followed by reverse-phase HPLC to separate [Leu] and [Met]enkephalin prior to assay, has assessed their concentrations as 59-170 pmol/l and 0.5-30 pmol/l, respectively, in three patients with chronic pain. The reproducibility of the HPLC separation step was checked by adding to the sample a synthetic analogue of very low cross-reactivity in UV detectable quantities. The assay procedure described is generally applicable to the assessment of [Leu] and [Met]enkephalin concentrations in CSF.

References

Sep 30, 1985·Biochemical and Biophysical Research Communications·F NybergL Terenius
Jan 1, 1970·Acta Endocrinologica. Supplementum·R Ekins, B Newman
Jul 1, 1981·International Journal of Peptide and Protein Research·D D Gay, R A Lahti
Feb 1, 1983·Journal of Neuroimmunology·P A PontarottiA A Cupo
Mar 1, 1982·The Journal of Clinical Investigation·T YoshimasaH Imura

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