PMID: 8971028Dec 1, 1996Paper

A specific subform of the human cytomegalovirus transactivator protein pUL69 is contained within the tegument of virus particles

Journal of Virology
M Winkler, T Stamminger

Abstract

The polypeptide encoded by the open reading frame UL69 of human cytomegalovirus (HCMV), which is homologous to the immediate-early regulator ICP27 of herpes simplex virus, has recently been identified as a transactivator protein that exerts a broad stimulatory effect on gene expression (M. Winkler, S. A. Rice, and T. Stamminger, J. Virol. 68:3943-3954, 1994). Here, we provide evidence that pUL69 is a phosphorylated tegument protein of HCMV. This finding could be demonstrated by Western blot (immunoblot) analyses with purified virions and a specific antiserum against pUL69. These experiments revealed that one phosphorylated subform of the three pUL69 polypeptides that are synthesized in infected fibroblast cells is contained within the HCMV virion. After the treatment of purified virions with detergents, pUL69 could not be detected within the membrane fraction, suggesting that it is either a capsid or a tegument protein. Its presence within dense bodies, however, shows that pUL69 is a constituent of the viral tegument.

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Citations

Dec 24, 2003·International Reviews of Immunology·Jonathan P Castillo, Timothy F Kowalik
Mar 30, 2005·Journal of Virology·Jill T BechtelDon Ganem
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mariko AoyagiThomas E Shenk
Feb 11, 2014·Viruses·Rebecca Marie SmithJulie Anne Kerry
Sep 5, 2002·Journal of Virology·Henri GruffatAlain Sergeant
Mar 1, 2006·Biotechnology and Bioengineering·Vy LamJohn Yin
Dec 14, 2016·The Journal of General Virology·Franz J J RiederChristoph Steininger
Sep 20, 2000·The Journal of General Virology·S K WangT T Chang
Aug 7, 2009·The Journal of General Virology·Shang-Kwei WangYu-Chang Tyan
Aug 7, 2007·Reviews in Medical Virology·Mark Bain, John Sinclair
Dec 3, 2003·The Journal of General Virology·Richard AdairAnamaris M Colberg-Poley
Sep 29, 2004·Journal of Virology·Lisa M KattenhornBenedikt M Kessler
Feb 12, 2002·Journal of Virology·Yingguang Liu, Bonita J Biegalke
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Robert F Kalejta
Mar 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·M L HayashiT Shenk
Jul 1, 1997·Journal of Virology·M J RomanowskiT Shenk

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