PMID: 9420331Feb 21, 1998Paper

A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm

Genes & Development
J F CáceresA R Krainer

Abstract

The SR proteins constitute a large family of nuclear phosphoproteins required for constitutive pre-mRNA splicing. These factors also have global, concentration-dependent effects on alternative splicing regulation and this activity is antagonized by members of the hnRNP A/B family of proteins. We show here that whereas some human SR proteins are confined to the nucleus, three of them-SF2/ASF, SRp20, and 9G8-shuttle rapidly and continuously between the nucleus and the cytoplasm. By swapping the corresponding domains between shuttling and nonshuttling SR proteins, we show that the carboxy-terminal arginine/serine-rich (RS) domain is required for shuttling. This domain, however, is not sufficient to promote shuttling of an unrelated protein reporter, suggesting that stable RNA binding mediated by the RNA-recognition motifs may be required for shuttling. Consistent with such a requirement, a double point-mutation in RRM1 of SF2/ASF that impairs RNA binding prevents the protein from shuttling. In addition, we show that phosphorylation of the RS domain affects the shuttling properties of SR proteins. These findings show that different SR proteins have unique intracellular transport properties and suggest that the family members that s...Continue Reading

References

Feb 20, 1992·Nature·S Piñol-Roma, G Dreyfuss
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·X D Fu, T Maniatis
May 1, 1992·Genes & Development·A M ZahlerM B Roth
Jul 10, 1992·Cell·U T Meier, G Blobel
Jan 1, 1983·Biology of the Cell·D L SpectorH Busch
Mar 1, 1994·The Journal of Cell Biology·A JarmolowskiI W Mattaj
Dec 22, 1994·Nature·I W Mattaj
Oct 15, 1995·Genes & Development·A C WilsonW Herr
Sep 15, 1995·The Journal of Biological Chemistry·P I DuncanJ C Bell
May 1, 1995·The Journal of Cell Biology·H Siomi, G Dreyfuss
Jun 8, 1995·Nature·M D Chiara, R Reed
Jul 1, 1994·Genes & Development·B E Meyer, M H Malim
Nov 1, 1994·The Journal of Cell Biology·B J BlencoweP A Sharp
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·X YangB Chabot
Jan 1, 1993·Annual Review of Cell Biology·D L Spector
Aug 13, 1993·Cell·M S Schmidt-ZachmannE A Nigg
Jan 1, 1993·Annual Review of Biochemistry·G DreyfussC G Burd
Aug 27, 1993·Cell·R A Laskey, C Dingwall
May 1, 1996·The Journal of Cell Biology·S Huang, D L Spector
Feb 1, 1996·Current Biology : CB·M S Moore
Jul 1, 1996·Genes & Development·J L Manley, R Tacke
Sep 20, 1996·Cell·V W PollardG Dreyfuss
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·W M MichaelG Dreyfuss
Oct 1, 1996·Molecular Biology of the Cell·T Misteli, D L Spector

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Citations

Aug 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K CzaplinskiS W Peltz
Aug 10, 2000·Archives of Biochemistry and Biophysics·Y AraoS Kato
Mar 10, 2000·Biochemical and Biophysical Research Communications·K SasaharaM Itakura
Mar 26, 2013·Chromosoma·Zhihong Zhou, Xiang-Dong Fu
Sep 4, 2012·Plant Molecular Biology·Koppolu Raja Rajesh Kumar, P B Kirti
Dec 5, 2003·Biochimica Et Biophysica Acta·Rebeca Martínez-ContrerasJesús Valdés
May 20, 2005·Current Opinion in Cell Biology·Robin Reed, Hong Cheng
Sep 27, 2000·Biochimica Et Biophysica Acta·Y SawadaS Ohtaki
Nov 10, 2000·Biochimica Et Biophysica Acta·H Jumaa, P J Nielsen
Feb 25, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·R L SlaytonM C Willing
Jun 26, 1998·Current Opinion in Cell Biology·T Misteli, D L Spector
Jun 26, 1998·Current Opinion in Cell Biology·J E Dahlberg, E Lund
Feb 1, 2000·Trends in Cell Biology·W M Michael
Aug 1, 2000·Trends in Biochemical Sciences·C W Smith, J Valcárcel
Jun 5, 2013·Chemical Reviews·C A NiñoC Dargemont
Apr 4, 2001·Traffic·M LichtensteinA M Tartakoff
Mar 13, 2013·Nature Reviews. Genetics·Michaela Müller-McNicoll, Karla M Neugebauer
Sep 6, 2007·Nature Reviews. Molecular Cell Biology·Alwin Köhler, Ed Hurt
Jul 20, 2010·Nature Structural & Molecular Biology·Minna-Liisa AnköKarla M Neugebauer
Jan 17, 2012·Nature Structural & Molecular Biology·Olga AnczukówAdrian R Krainer
Aug 6, 2008·Nature Structural & Molecular Biology·Juan Pablo Fededa, Alberto R Kornblihtt
Nov 22, 2005·Nature Structural & Molecular Biology·Matías BlausteinAnabella Srebrow
Feb 21, 2007·Nature Structural & Molecular Biology·Rotem KarniAdrian R Krainer
Aug 4, 2005·Nature Structural & Molecular Biology·Ravinder Singh, Juan Valcárcel
Oct 6, 2009·The Journal of Biological Chemistry·Alejandro Cassola, Alberto C Frasch
Sep 28, 2010·The Journal of Biological Chemistry·Aparna DixitPushkar Sharma
Nov 6, 2002·The Journal of Experimental Medicine·Makoto KamachiPaul J Utz
Oct 8, 1999·Molecular Biology of the Cell·M Bellini, J G Gall
Jan 21, 2012·Molecular Biology of the Cell·Noriko SaitohMitsuyoshi Nakao
Dec 4, 2010·Briefings in Functional Genomics·Shivendra KishoreMihaela Zavolan
Jun 25, 2010·Glycobiology·Kevin C HaudekJohn L Wang

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