A spectrophotometric method for the assay of pyrimidine 5'-nucleotidase in human erythrocytes

British Journal of Haematology
A AmiciG Magni

Abstract

A new spectrophotometric assay for human erythrocyte pyrimidine 5'-nucleotidase using a continuous spectrophotometric method is described. The activity is detected by measuring uracil, which is produced by a coupled assay including cytidine deaminase and uridine nucleosidase. The results obtained with the continuous spectrophotometric method have been confirmed by high pressure liquid chromatography (HPLC) analysis. This procedure, not based upon inorganic phosphate formation and avoiding the use of radioactive material, offers significant advantages with respect to commonly adopted assays.

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J D TorranceE Beutler
Jan 1, 1978·Methods in Enzymology·G Magni
Jan 1, 1978·Methods in Enzymology·P L Ipata, G Cercignani
Apr 17, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·H Buc, J C Kaplan
Aug 15, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·P H EllimsM B Van der Weyden
Dec 1, 1985·Analytical Biochemistry·C R Zerez, K R Tanaka
Oct 1, 1974·The Journal of Clinical Investigation·W N ValentineW S Adams
Nov 1, 1982·Analytical Biochemistry·J D TorranceW Mills

Related Concepts

Uridine nucleosidase
High Pressure Liquid Chromatography Procedure
Cytidine Deaminase
Erythrocytes
Hydrogen-Ion Concentration
Nucleosidases
Spectrophotometry
Magnesium Chloride
5'-NUCLEOTIDASE

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