A spectroscopic method to estimate the binding potency of amphiphile assemblies

Analytical and Bioanalytical Chemistry
D R GaugerW Pohle

Abstract

A fast and convenient spectroscopic methodology to determine the water uptake capacity of amphiphile assemblies studied in multilayer films is presented. This method was developed to provide a reliable but relatively simple tool for estimating the binding potency of such complex systems. The water-binding potency represents a general propensity of higher-order systems to bind or embed relevant ligands, such as various non-lipid effectors in the case of artificial lipid membranes. In this sense, the binding potency might contribute to a specific functional role of certain lipids. The essence of the new method is that the calibration of data measured by infrared (IR) spectroscopy against those directly obtained by Karl-Fischer titration (KFT) enables one to replace the expensive chemical-analytical technique by a more comfortable and efficient IR-spectroscopic protocol. This approach combines the easy handling, versatility, and availability of IR spectroscopy with the high accuracy of KFT. The usefulness of the procedure is demonstrated on an example set of six amphiphiles with a common chain length of 18 carbon atoms. Despite this similarity, the binding potency data differ tremendously in a way which can be correlated with the ...Continue Reading

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Citations

Sep 21, 2011·The Journal of Physical Chemistry. a·Nancy E LevingerThomas Elsaesser
May 18, 2019·Physical Chemistry Chemical Physics : PCCP·Valery Andrushchenko, Walter Pohle
Feb 19, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Anna V TugarovaAlexander A Kamnev

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