PMID: 6969292Nov 1, 1980Paper

A spectroscopic study of metal ion and ligand binding to beta-lactamase II

Journal of Inorganic Biochemistry
G S BaldwinE P Abraham

Abstract

beta-Lactamase II has two metal-binding sites. The electronic spectra of Cd(II)- and Co(II)-substituted beta-lactamase II have been investigated. It is suggested that a thiol ligand is involved in metal binding at the first site. The stoichiometric dissociation constants for Co(II) binding to beta-lactamase II were estimated to be 0.13 and 2.66 mM (pH 6.0, 4 degrees C, 1 M NaCl) by equilibrium dialysis. Competition between Zn(II) and Co(II) for the first metal binding site suggests a value of 0.7 microM (pH 6.0, 30 degrees C, 1 M NaCl) for the dissociation constant of Zn(II). The electronic spectra of the Co(II) enzyme lead to the suggestion that the coordination geometries around the metal ions in the first and second sites are related to those of a distorted tetrahedron and octahedron, respectively.

References

Nov 1, 1978·The Biochemical Journal·G S BaldwinE P Abraham
Jul 25, 1978·Journal of Molecular Biology·E T AdmanL H Jensen
Mar 25, 1976·Journal of Molecular Biology·H EklundA Akeson
Feb 3, 1979·British Medical Journal·K Dewhurst
Aug 8, 1978·Biochemistry·S W May, J Y Kuo
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·K K KannanM Petef
Dec 15, 1975·Biochemical and Biophysical Research Communications·A J Sytkowski, B L Vallee
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D R McMillinH B Gray
Oct 1, 1974·The Biochemical Journal·R B Davies, E P Abraham
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D R McMillinH B Gray
Oct 9, 1970·Biochemical and Biophysical Research Communications·D E Drum, B L Vallee
Jan 1, 1972·Cold Spring Harbor Symposia on Quantitative Biology·K D WatenpaughL H Jensen
Jan 1, 1973·Advances in Experimental Medicine and Biology·B L Vallee
Jan 1, 1966·The Biochemical Journal·L D Sabath, E P Abraham
Jun 1, 1980·The Biochemical Journal·A GaldesE P Abraham
Jan 1, 1957·Methods of Biochemical Analysis·R E THIERS

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Citations

Jan 1, 1987·Progress in Biophysics and Molecular Biology·S S Hasnain, C D Garner
Apr 21, 1999·Protein Science : a Publication of the Protein Society·Z LiO Herzberg
Nov 1, 1986·Antimicrobial Agents and Chemotherapy·G J CuchuralF P Tally
Jun 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Qi-Zhuang YeRobert P Hanzlik
Nov 19, 1985·Biochemistry·R Bicknell, S G Waley
Feb 9, 1988·Biochemistry·R BicknellD S Auld
Sep 28, 2017·Physical Chemistry Chemical Physics : PCCP·Elena DecanetoNicholas Cox
Sep 21, 2001·The Journal of Biological Chemistry·C MollardG C Roberts
May 1, 1999·The Journal of Biological Chemistry·R Paul-SotoH W Adolph
Oct 1, 1982·Archives of Biochemistry and Biophysics·J HiroseY Kidani

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