A squid dynein isoform promotes axoplasmic vesicle translocation.

The Journal of Cell Biology
S P Gilbert, R D Sloboda

Abstract

Axoplasmic vesicles that translocate on isolated microtubules in an ATP-dependent manner have an associated ATP-binding polypeptide with a previously estimated relative molecular mass of 292 kD (Gilbert, S. P., and R. D. Sloboda. 1986. J. Cell Biol. 103:947-956). Here, data are presented showing that this polypeptide (designated H1) and another high molecular mass polypeptide (H2) can be isolated in association with axoplasmic vesicles or optic lobe microtubules. The H1 and H2 polypeptides dissociate from microtubules in the presence of MgATP and can be further purified by gel filtration chromatography. The peak fraction thus obtained demonstrates MgATPase activity and promotes the translocation of salt-extracted vesicles (mean = 0.87 microns/s) and latex beads (mean = 0.92 microns/s) along isolated microtubules. The H1 polypeptide binds [alpha 32P]8-azidoATP and is thermosoluble, but the H2 polypeptide does not share these characteristics. In immunofluorescence experiments with dissociated squid axoplasm, affinity-purified H1 antibodies yield a punctate pattern that corresponds to vesicle-like particles, and these antibodies inhibit the bidirectional movement of axoplasmic vesicles. H2 is cleaved by UV irradiation in the prese...Continue Reading

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R D SlobodaP Greengard
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1979·The Journal of Cell Biology·H KimJ L Rosenbaum
Aug 15, 1977·European Journal of Biochemistry·A FellousJ Nunez
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D B Murphy, G G Borisy
Oct 1, 1985·The Journal of Cell Biology·W S SaleJ E Heuser
Jun 20, 1987·Journal of Molecular Biology·S Hisanaga, N Hirokawa
Jan 1, 1987·Cell Motility and the Cytoskeleton·S P Marchese-RagonaK A Johnson
Jan 1, 1988·Cell Motility and the Cytoskeleton·A T HastieJ S Wall
Jan 1, 1987·Annual Review of Cell Biology·R D Vale
Nov 12, 1987·Nature·B M Paschal, R B Vallee
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M L ShelanskiC R Cantor
Feb 1, 1973·Analytical Biochemistry·G R Schacterle, R L Pollack
Dec 1, 1981·The Journal of Cell Biology·S R HeidemannM A Hamborg
Jan 1, 1984·Journal of Cellular Biochemistry·M M PrattD A Begg
Mar 1, 1983·The Journal of Cell Biology·K A Johnson, J S Wall
Dec 25, 1984·Journal of Molecular Biology·U Goodenough, J Heuser
Oct 1, 1984·Neuroscience·N HaghighatG D Pappas
Jan 1, 1983·Journal of Microscopy·R D Allen, N S Allen

❮ Previous
Next ❯

Citations

Jan 1, 1994·Cell Motility and the Cytoskeleton·S A KuznetsovG M Langford
Jan 1, 1992·Biology of the Cell·I R GibbonsB H Gibbons
Dec 1, 1991·The Journal of Cell Biology·T A Schroer, M P Sheetz
Apr 1, 1994·Brain Pathology·J A MercerS T Brady
Mar 10, 1997·The Journal of Cell Biology·B SodeikA Helenius
Feb 1, 1991·Current Opinion in Cell Biology·T A Schroer
Apr 23, 1992·Nature·S A KuznetsovD G Weiss
Oct 1, 1991·Neuron·S T Brady

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.