A stable Escherichia coli-mycobacteria shuttle vector 'pSO246' in Mycobacterium bovis BCG

FEMS Microbiology Letters
S MatsumotoT Yamada

Abstract

The most widely used plasmid vector system in mycobacteria is based on pAL5000 from Mycobacterium fortuitum. The derivatives of the pAL5000-based shuttle vectors between Escherichia coli and mycobacteria, which we have utilized to secrete recombinant antigens, were generated. The stability of the vectors was assessed in Mycobacterium bovis BCG (BCG). The plasmid vector pSO246 was stable in BCG for at least 50 generations.

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D E BergJ D Rochaix
Jan 2, 1992·Gene·S Hinshelwood, N G Stoker
Jun 6, 1991·Nature·C K StoverG F Hatfull
Sep 1, 1989·Tubercle·L LugosiB R Bloom
Sep 1, 1985·Annales De L'Institut Pasteur. Microbiology·A LabidiD Roulland-Dussoix

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Citations

Sep 18, 2007·Journal of Bacteriology·Tomoya KatsubeKazuo Kobayashi
Jan 5, 2012·Nihon saikingaku zasshi. Japanese journal of bacteriology·Sohkichi Matsumoto

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