A stable explant culture of HER2/neu invasive carcinoma supported by alpha-Smooth Muscle Actin expressing stromal cells to evaluate therapeutic agents.

BMC Cancer
Marie P Piechocki

Abstract

To gain a better understanding of the effects of therapeutic agents on the tumor microenvironment in invasive cancers, we developed a co-culture model from an invasive lobular carcinoma. Tumor cells expressing HER2/neu organize in nests surrounded by alpha-Smooth Muscle Actin (alpha-SMA) expressing tumor stroma to resemble the morphology of an invading tumor. This co-culture, Mammary Adenocarcinoma Model (MAM-1) maintains a 1:1 ratio of HER2/neu positive tumor cells to alpha-SMA-reactive stromal cells and renews this configuration for over 20 passages in vitro. We characterized the cellular elements of the MAM-1 model by microarray analysis, and immunocytochemistry. We developed flow cytometric assays to evaluate the relative responses of the tumor and stroma to the tyrosine kinase inhibitor, Iressa. The MAM-1 gene expression profile contains clusters that represent the ErbB-2 breast cancer signature and stroma-specific clusters associated with invasive breast cancers. The stability of this model and the ability to antigenically label the tumor and stromal fractions allowed us to determine the specificity of Iressa, a receptor tyrosine kinase inhibitor, for targeting the tumor cell population. Treatment resulted in a selective ...Continue Reading

References

Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Mar 28, 1997·International Journal of Cancer. Journal International Du Cancer·X Dong-Le BourhisF Calvo
Aug 24, 1999·Pharmacology & Therapeutics·J R Woodburn
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·T SørlieA L Børresen-Dale
Sep 28, 2001·In Vitro Cellular & Developmental Biology. Animal·C S WangB Têtu
Jun 12, 2003·Methods : a Companion to Methods in Enzymology·Thorarinn GudjonssonOle William Petersen
Jan 27, 2004·Oncogene·François BertucciDaniel Birnbaum
Mar 23, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hélène FrançoisChristos Chatziantoniou
Aug 6, 2004·The European Respiratory Journal·T InoueK Yamaguchi
Dec 2, 2004·Nature Reviews. Cancer·Roy S HerbstJosé Baselga
May 3, 2005·Nature Reviews. Cancer·Nancy E Hynes, Heidi A Lane
May 5, 2005·PLoS Biology·Robert B WestMatt van de Rijn
Nov 23, 2005·Expert Opinion on Therapeutic Targets·Patrick Micke, Arne Ostman
Feb 14, 2006·International Journal of Cancer. Journal International Du Cancer·Marie P PiechockiFulvio Lonardo
Jun 3, 2006·American Journal of Respiratory and Critical Care Medicine·Yoshiki IshiiTakeshi Fukuda
Jun 16, 2006·Journal of the American Society of Nephrology : JASN·Bogdan BeirowskiOliver Gross
Jul 13, 2006·Breast Cancer Research : BCR·Magnus D LynchAnand D Purushotham
Sep 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Norihiko SakaiShuichi Kaneko
Jan 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kwang S SuhStuart H Yuspa
Feb 23, 2007·International Journal of Cancer. Journal International Du Cancer·Yuejuan LiParaskevi Heldin
Mar 30, 2007·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Chun ZhangAnguo Deng
Dec 14, 2007·International Journal of Cancer. Journal International Du Cancer·Marie P PiechockiGeorge H Yoo
Aug 14, 2008·Expert Review of Anticancer Therapy·Massimiliano Cariati

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Citations

Jun 18, 2009·In Vitro Cellular & Developmental Biology. Animal·Nirmala Parajuli, Wolfgang Doppler
Feb 20, 2018·Molecular Cancer·Hor-Yue TanYung-Chi Cheng
May 4, 2013·Experimental Lung Research·Olivier BoucheratChristophe Delacourt

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Datasets Mentioned

BETA
MAM-1

Methods Mentioned

BETA
flow cytometry
Transgenic
FACS
chips

Software Mentioned

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