A stable isotope dilution assay for the antiparkinsonian drug benztropine in biological fluids

Clinica Chimica Acta; International Journal of Clinical Chemistry
S P JindalP Vestergaard

Abstract

A quantitative gas chromatographic-mass spectrometric (GC-MS) assay was developed for the determination of benztropine in human urine and plasma. The assay utilizes selected ion focusing to monitor in a GC effluent a specific fragment ion of benztropine generated by electron-impact ionization. Benztropine-d3 was the internal standard. The assay can measure 5 ng/ml of benztropine/ml with about 6% precision. The curve relating the amounts of benztropine added versus the amounts found over a large range of benztropine concentrations was a straight line with a nearly zero intercept and a slope of 0.98 +/- 0.02. The method was used for the analysis of benztropine in urine and plasma of patients on therapeutic dose of the drug.

References

Jul 1, 1976·The American Journal of Psychiatry·G Chouinard, L Annable
Mar 1, 1975·Journal of Medicinal Chemistry·R N Booher, A Pohland
Mar 1, 1976·British Journal of Pharmacology·L Rivera-Calimlim

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Citations

Jun 13, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·R E Hill, D T Whelan
Apr 5, 2014·Journal of Forensic Sciences·Iain M McIntyreJacquelyn Morhaime
Oct 1, 1993·Journal of Pharmaceutical Sciences·G D Mellick, M S Roberts
Feb 1, 2002·International Journal of Epidemiology·P GrandjeanU Steuerwald
Aug 1, 1995·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·H HeK K Midha
May 10, 1985·Journal of Chromatography·V SchwarzK Macek

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