A stable-isotope dilution GC-MS approach for the analysis of DFRC (derivatization followed by reductive cleavage) monomers from low-lignin plant materials

Journal of Agricultural and Food Chemistry
Judith SchäferMirko Bunzel

Abstract

The derivatization followed by reductive cleavage (DFRC) method is a well-established tool to characterize the lignin composition of plant materials. However, the application of the original procedure, especially the chromatographic determination of the DFRC monomers, is problematic for low-lignin foods. To overcome these problems a modified sample cleanup and a stable-isotope dilution approach were developed and validated. To quantitate the diacetylated DFRC monomers, their corresponding hexadeuterated analogs were synthesized and used as internal standards. By using the selected-ion monitoring mode, matrix-associated interferences can be minimized resulting in higher selectivity and sensitivity. The modified method was applied to four low-lignin samples. Lignin from carrot fibers was classified as guaiacyl-rich whereas the lignins from radish, pear, and asparagus fibers where classified as balanced lignins (guaiacyl/syringyl ratio=1-2).

References

Mar 8, 1996·Chemico-biological Interactions·P J HarrisL R Ferguson
Feb 7, 2001·Journal of Agricultural and Food Chemistry·J PengJ Ralph
Sep 25, 2003·Annual Review of Plant Biology·Wout BoerjanMarie Baucher
Apr 28, 2005·Journal of Agricultural and Food Chemistry·Hoon Kim, John Ralph
Nov 24, 2005·Journal of Agricultural and Food Chemistry·Mirko BunzelHans Steinhart
Mar 2, 2006·Journal of Agricultural and Food Chemistry·Carola FunkMirko Bunzel
Oct 13, 2006·Journal of Agricultural and Food Chemistry·Mirko Bunzel, John Ralph
Apr 24, 2013·Nutrients·Joanne Slavin

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Citations

Nov 3, 2016·Journal of Agricultural and Food Chemistry·Judith SchäferMirko Bunzel
Nov 4, 2016·The Plant Journal : for Cell and Molecular Biology·Wu LanJohn Ralph
Jul 19, 2017·Journal of the Science of Food and Agriculture·Judith SchäferMirko Bunzel
Jul 23, 2021·Mass Spectrometry Reviews·Dane R Letourneau, Dietrich A Volmer

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