A stable RNA G-quadruplex within the 5'-UTR of Arabidopsis thaliana ATR mRNA inhibits translation

The Biochemical Journal
Chun Kit KwokPhilip C Bevilacqua

Abstract

Guanine quadruplex structures (GQSs) play important roles in the regulation of gene expression and cellular processes. Recent studies provide strong evidence for the formation and function of DNA and RNA GQSs in human cells. However, whether GQSs form and are functional in plants remains essentially unexplored. On the basis of circular dichroism (CD)-detected titration, UV-detected melting, in-line probing (ILP) and reporter gene assay studies, we report the first example of a plant RNA GQS that inhibits translation. This GQS is located within the 5'-UTR of the ATAXIA TELANGIECTASIA-MUTATED AND RAD3-RELATED (ATR) mRNA of Arabidopsis thaliana (mouse-ear cress). We show that this GQS is highly stable and is thermodynamically favoured over a competing hairpin structure in the 5'-UTR at physiological K⁺ and Mg²⁺ concentrations. Results from ILP reveal the secondary structure of the RNA and support formation of the GQS in vitro in the context of the complete 5'-UTR. Transient reporter gene assays performed in living plants reveal that the GQS inhibits translation but not transcription, implicating this GQS as a translational repressor in vivo. Our results provide the first complete demonstration of the formation and function of a re...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N SonenbergA J Shatkin
Jan 1, 1989·Methods in Enzymology·J F Milligan, O C Uhlenbeck
Oct 2, 1998·FEBS Letters·J L MergnyL Lacroix
Jul 8, 2000·The Plant Journal : for Cell and Molecular Biology·Y YangM Qi
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
Aug 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Adam Siddiqui-JainLaurence H Hurley
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
May 26, 2005·Nucleic Acids Research·Julian L Huppert, Shankar Balasubramanian
Oct 6, 2005·Biochemistry·J Kenneth WickiserDonald M Crothers
Jul 18, 2006·Nucleic Acids Research·Oleg KikinParamjeet S Bagga
Feb 27, 2007·Nature Chemical Biology·Sunita KumariShankar Balasubramanian
Apr 3, 2007·FEBS Letters·Phillip A RachwalKeith R Fox
Jul 28, 2007·Chemistry & Biology·Markus Wieland, Jörg S Hartig
Nov 30, 2007·Nucleic Acids Research·Oleg KikinParamjeet S Bagga
Apr 2, 2008·Methods in Molecular Biology·Jeffrey Wilusz
Jul 3, 2008·Nature Reviews. Molecular Cell Biology·Karlene A Cimprich, David Cortez
Oct 4, 2008·Nucleic Acids Research·Julian Leon HuppertShankar Balasubramanian
Jan 16, 2009·BMC Genomics·Damian SmedleyArek Kasprzyk
Mar 28, 2009·Nucleic Acids Research·Joseph D SchonhoftSoumitra Basu
Jun 24, 2009·Biopolymers·Miguel Angel Mendez, Veronika A Szalai
Feb 12, 2010·Nucleic Acids Research·Gaëlle PennarunFrançois D Boussin
Mar 5, 2010·Current Protocols in Nucleic Acid Chemistry·Yuta Sannohe, Hiroshi Sugiyama
Mar 27, 2010·Molecular Plant·Yi Wang, Wei-Hua Wu
Jun 18, 2010·Methods : a Companion to Methods in Enzymology·Justin T Low, Kevin M Weeks
Jun 24, 2010·Biochimie·Mireille Melko, Barbara Bardoni
Jun 24, 2010·Nucleic Acids Research·Dennis GomezPatrick Calsou
Jun 24, 2010·Nucleic Acids Research·Jean-Denis Beaudoin, Jean-Pierre Perreault
Jul 28, 2010·Nucleic Acids Research·Aurore GuédinJean-Louis Mergny
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Paulina H WanrooijClaes M Gustafsson
Sep 24, 2010·Nucleic Acids Research·Melissa A MullenPhilip C Bevilacqua
Nov 26, 2010·Journal of the American Chemical Society·Mark J MorrisSoumitra Basu
May 14, 2011·EMBO Reports·Murugan SubramanianHervé Moine

❮ Previous
Next ❯

Citations

Feb 21, 2016·Database : the Journal of Biological Databases and Curation·Subodh Kumar Mishra, Amit Kumar
Oct 24, 2015·The Plant Journal : for Cell and Molecular Biology·Maxwell S BushJohn H Doonan
Dec 1, 2015·PloS One·Ezekiel CrenshawAleister J Saunders
Jun 9, 2016·The Journal of Physical Chemistry. B·Madeline E SherlockPhilip C Bevilacqua
Jul 30, 2016·RNA Biology·Kristina SzameitUlrich Hahn
Aug 30, 2016·Nature Methods·Chun Kit KwokShankar Balasubramanian
Sep 27, 2016·Frontiers in Chemistry·Debmalya BhattacharyyaSoumitra Basu
Jun 1, 2018·Annual Review of Microbiology·H Steven Seifert
Sep 12, 2018·Chemical Communications : Chem Comm·Ka Lung ChanChun Kit Kwok
Jan 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhi-Yin SunTian-Miao Ou
Dec 3, 2016·Biochemical Society Transactions·Chun Kit Kwok
Nov 14, 2019·Nucleic Acids Research·Yueying ZhangYiliang Ding
Nov 23, 2019·Nucleic Acids Research·Emilia Puig Lombardi, Arturo Londoño-Vallejo
Oct 24, 2019·Animal Biotechnology·Georgios C StefosIoannis Politis
Sep 26, 2020·Nucleic Acids Research·Mubarak I Umar, Chun Kit Kwok
Apr 25, 2015·Angewandte Chemie·Chun Kit Kwok, Shankar Balasubramanian
May 6, 2020·Wiley Interdisciplinary Reviews. RNA·Ricardo A Urquidi CamachoAlbrecht G von Arnim
May 29, 2018·Nature Plants·Yiliang Ding, Chun Kit Kwok
Sep 3, 2020·Genome Biology·Xiaofei YangYiliang Ding
Jun 7, 2018·Frontiers in Plant Science·Xiaofei YangYiliang Ding
Jul 19, 2019·Frontiers in Plant Science·Piotr M Kopec, Wojciech M Karlowski
Jun 30, 2016·Angewandte Chemie·Chun Kit KwokShankar Balasubramanian
Jul 21, 2017·Frontiers in Plant Science·Vikas YadavPuja Yadav
Dec 18, 2020·Frontiers in Cell and Developmental Biology·Alexandra A KuznetsovaNikita A Kuznetsov
Mar 28, 2021·Nucleic Acids Research·Kaixin LyuChun Kit Kwok
Nov 16, 2017·Journal of Medicinal Chemistry·Giulia MigliettaLuigi E Xodo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.

Ataxia telangiectasia

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.