PMID: 6170121May 1, 1981Paper

A staining procedure for identifying viable cell hybrids constructed by somatic cell fusion, cybridization, or nuclear transplantation

Somatic Cell Genetics
M J HightowerJ J Lucas

Abstract

A general procedure for identifying viable hybrid cells was developed. One cell type was labeled by a brief incubation in the Kodak laser dye rhodamine 123, which accumulates in the mitochondria; a second cell type was labeled by a brief incubation in the Hoechst fluorochrome 33258, which binds to chromatin. The substances which are eventually lost from the organelles, appeared to be nontoxic; the plating efficiencies of numerous cell lines tested was unaffected. Either whole cells or cytoplasts labeled with rhodomine 123 were fused, using inactivated Sendai virus, to whole cells or karyoplasts labeled with Hoechst 33258. When living cells were illuminated with ultraviolet light, individual whole cell hybrids, cybrids or cytoplasmic- nuclear hybrid cells could be rapidly identified by the appropriate staining pattern.

References

Sep 1, 1975·The Journal of Cell Biology·F G MoserF H Ruddle
Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D J Arndt-Jovin, T M Jovin
Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S A Latt
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·U KrondahlN R Ringertz
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G VeomettK R Porter
Feb 1, 1980·Journal of Cellular Physiology·M J LydonD B Thomas
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L V JohnsonL B Chen
Oct 1, 1980·Journal of Cellular Physiology·M J Hightower, J J Lucas

❮ Previous
Next ❯

Citations

May 1, 1985·Somatic Cell and Molecular Genetics·Y Matsuya, I Yamane
May 1, 1983·Somatic Cell Genetics·T Kliot-FieldsA Wiseman
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·I C SummerhayesL B Chen
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M J HightowerJ J Lucas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.