PMID: 8610119Apr 2, 1996Paper

A stationary-phase stress-response sigma factor from Mycobacterium tuberculosis

Proceedings of the National Academy of Sciences of the United States of America
J DeMaioW R Bishai

Abstract

Alternative RNA polymerase sigma factors are a common means of coordinating gene regulation in bacteria. Using PCR amplification with degenerate primers, we identified and cloned a sigma factor gene, sigF, from Mycobacterium tuberculosis. The deduced protein encoded by sigF shows significant similarity to SigF sporulation sigma factors from Streptomyces coelicolor and Bacillus subtilis and to SigB, a stress-response sigma factor, from B. subtilis. Southern blot surveys with a sigF-specific probe identified cross-hybridizing bands in other slow-growing mycobacteria, Mycobacterium bovis bacille Calmette-Guérin (BCG) and Mycobacterium avium, but not in the rapid-growers Mycobacterium smegmatis or Mycobacterium abscessus. RNase protection assays revealed that M. tuberculosis sigF mRNA is not present during exponential-phase growth in M. bovis BCG cultures but is strongly induced during stationary phase, nitrogen depletion, and cold shock. Weak expression of M. tuberculosis sigF was also detected during late-exponential phase, oxidative stress, anaerobiasis, and alcohol shock. The specific expression of M. tuberculosis sigF during stress or stationary phase suggests that it may play a role in the ability of tubercle bacilli to adapt...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R SchmidtR Losick
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A K Benson, W G Haldenwang

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Citations

Mar 8, 2008·Current Microbiology·Anirudh K Singh, Bhupendra N Singh
Jul 29, 2000·FEMS Microbiology Letters·B Hutter, T Dick
Jun 18, 2002·Research in Microbiology·Siobhán C CowleyYossef Av-Gay
Aug 11, 2001·Current Opinion in Immunology·B Raupach, S H Kaufmann
Jan 1, 1997·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·J DeMaioW R Bishai
Apr 29, 2000·Trends in Microbiology·Y Av-Gay, M Everett
Jan 1, 1997·Molecular Medicine Today·D B Young
Aug 28, 1998·Molecular Microbiology·M GomezI Smith
Feb 23, 1999·Molecular Microbiology·R ManganelliI Smith
Dec 20, 2002·Seminars in Pediatric Infectious Diseases·Paul F Riska, Stephen Carleton
Oct 18, 2002·Nucleic Acids Research·Adel M TalaatStephen Albert Johnston
Jul 15, 2003·Clinical Microbiology Reviews·Issar Smith
Nov 13, 2007·Journal of Bacteriology·Jong-Hee LeeWilliam R Bishai
Feb 7, 2012·Journal of Bacteriology·Ruben C HartkoornStewart T Cole
Nov 28, 2000·Journal of Bacteriology·S GertzM Hecker
Mar 29, 2001·Journal of Bacteriology·C BoonT Dick
Nov 26, 2002·Journal of Bacteriology·Calvin Boon, Thomas Dick
Jun 19, 2004·Journal of Bacteriology·Markus BischoffSteven Projan
Feb 6, 2004·Journal of Bacteriology·Riccardo ManganelliIssar Smith
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Mark J KazmierczakKathryn J Boor
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·Ying Zhang

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