A Statistical Framework for Mapping Risk Genes from De Novo Mutations in Whole-Genome-Sequencing Studies.

American Journal of Human Genetics
Yuwen LiuXin He

Abstract

Analysis of de novo mutations (DNMs) from sequencing data of nuclear families has identified risk genes for many complex diseases, including multiple neurodevelopmental and psychiatric disorders. Most of these efforts have focused on mutations in protein-coding sequences. Evidence from genome-wide association studies (GWASs) strongly suggests that variants important to human diseases often lie in non-coding regions. Extending DNM-based approaches to non-coding sequences is challenging, however, because the functional significance of non-coding mutations is difficult to predict. We propose a statistical framework for analyzing DNMs from whole-genome sequencing (WGS) data. This method, TADA-Annotations (TADA-A), is a major advance of the TADA method we developed earlier for DNM analysis in coding regions. TADA-A is able to incorporate many functional annotations such as conservation and enhancer marks, to learn from data which annotations are informative of pathogenic mutations, and to combine both coding and non-coding mutations at the gene level to detect risk genes. It also supports meta-analysis of multiple DNM studies, while adjusting for study-specific technical effects. We applied TADA-A to WGS data of ∼300 autism-affected...Continue Reading

Citations

Jul 16, 2020·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Veronica B Searles QuickMatthew W State
Jun 6, 2020·Human Genetics·Ozvan Bocher, Emmanuelle Génin
Oct 9, 2018·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Dimuthu AlankarageSally L Dunwoodie
May 27, 2021·Nature Communications·Zihuai HeIuliana Ionita-Laza

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