Apr 1, 2020

Single-molecule imaging reveals translation of mRNAs localized to stress granules

BioRxiv : the Preprint Server for Biology
D. MatejuJeffrey Alan Chao

Abstract

Cellular stress leads to reprogramming of mRNA translation and formation of stress granules (SGs), membrane-less organelles consisting of mRNA and RNA-binding proteins. Although the function of SGs remains largely unknown, it is widely assumed they contain exclusively non-translating mRNA. Here we re-examine this hypothesis using single-molecule imaging of mRNA translation in living cells. While our data confirms that non-translating mRNAs are preferentially recruited to SGs, we find unequivocal evidence for translation of mRNA localized to SGs. Our data indicate that SG-associated translation is not rare and that the entire translation cycle (initiation, elongation and termination) can occur for these transcripts. Furthermore, translating mRNAs can be observed transitioning between the cytosol and SGs without changing their translational status. Together, these results argue against a direct role for SGs in inhibition of mRNA translation.

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Mentioned in this Paper

Positioning Attribute
Genome
Human Genetics
Genomics
Gene Annotation
Conservative Therapy
Learning
Analysis
High-Throughput RNA Sequencing
Whole Genome Sequencing

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