PMID: 6963882Jan 1, 1982Paper

A stereologic analysis of collagen phagocytosis by periodontal ligament fibroblasts during occlusal hypofunction in the rat

Archives of Oral Biology
D A DeporterT P Howley

Abstract

Maxillary molars were extracted unilaterally from 4 young male rats so as to leave the agonist mandibular molars in hypofunction. The mandibular molars on the opposite side of the mouth acted as controls. Three days later, the animals were killed by perfusion and all mandibular molars removed en bloc. After demineralization, each block was trimmed, prepared for electron microscopy and embedded in Epon. The Epon blocks were trimmed so as to comprise the middle-third of the buccal periodontal ligament of the mesio-buccal root of the first molar together with some cementum and alveolar bone as landmarks. Two light-gold sections were cut from each trimmed block and systematically photographed in the electron microscope. The photographs were analysed stereologically so as to quantitate phagocytosed collagen (ICC), cytoplasm (CC) and extracellular fibrillar collagen (ECC). The results for the hypofunctional and contralateral control ligaments were compared with each other and with earlier results from similar but untreated (baseline) animals. The results were expressed as mean values for ICC, CC and ECC, and as the ratios ICC/ECC (the fraction of extracellular collagen phagocytosed), ICC/CC (collagen phagocytic activity per unit cyto...Continue Reading

Citations

Nov 12, 2013·European Journal of Oral Sciences·Balazs J DenesStavros Kiliaridis
Jun 21, 2018·The Journal of International Medical Research·Domna DorotheouStavros Kiliaridis

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