A stereological approach for estimation of cellular immunogold labeling and its spatial distribution in oriented sections using the rotator.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
John Lucocq, Christian Gawden-Bone

Abstract

Particulate gold labeling applied to ultrathin sections is a powerful approach for locating cellular proteins and lipids on thin sections of cellular structures and compartments. Effective quantitative methods now allow estimation of both density and distribution of gold labeling across aggregate organelles or compartment profiles. However, current methods generally use random sections of cells and tissues, and these do not readily present the information needed for spatial mapping of cellular quantities of gold label. Yet spatial mapping of gold particle labeling becomes important when cells are polarized or show internal organization or spatial shifts in protein/lipid localization. Here we have applied a stereological approach called the rotator to estimate cellular gold label and proportions of labeling over cellular compartments at specific locations related to a chosen cell axis or chosen cellular structures. This method could be used in cell biology for mapping cell components in studies of protein translocation, cell polarity, cell cycle stages, or component cell types in tissues.

References

Dec 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Lucocq
Jul 1, 1988·Journal of Microscopy·H J Gundersen
Oct 1, 1984·Journal of Ultrastructure Research·G GriffithsJ Dubochet
May 1, 1997·Journal of Microscopy·T TandrupE B Jensen
Mar 10, 2001·Science·M Bendayan
Apr 29, 2005·Journal of Anatomy·Simon McCullough, John Lucocq
Jul 29, 2006·Journal of Microscopy·J R Nyengaard, H J G Gundersen
Apr 5, 2008·Journal of Microscopy·F D'Amico, E Skarmoutsou
Jun 17, 2008·Histochemistry and Cell Biology·Terry M Mayhew, John M Lucocq

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