A Stereological Study of Mouse Ovary Tissues for 3D Bioprinting Application.

Cellular and Molecular Bioengineering
Jia-Hua ZhengXianghua Huang

Abstract

The use of 3D-bioprinted ovaries has been proven to be a promising technique for preserving fertility. Stereology is an accurate method to obtain quantitative 3D information and the stereological data is the basis for 3D bioprinting ovaries. In this study, six female mice were used to acquire the ovarian tissues. One of the two paraffin-embedded ovaries of each mouse was cut into 5 µm sections, and the other was cut into 15 µm sections and then subjected to haematoxylin and eosin staining and anti-follicle stimulating hormone receptor antibody immunohistochemistry. The volume and volume fractions of ovaries were measured by the Cavalieri method. Then, the numerical densities and total numbers of ovarian granulosa cells (OGCs) and primordial, preantral and antral follicles in serial sections were estimated using design-based stereology. The ovarian volume was 2.50 ± 0.32 mm3. The volume fractions of the cortex, medulla, follicles and OGCs were 86.80% ± 2.82, 13.20% ± 2.82%, 5.60% ± 0.25% and 81.19% ± 2.57%, respectively. The numerical densities of OGCs, the primordial, preantral and antral follicles were 2.11 (± 0.28) × 106/mm3, 719.57 ± 18.04/mm3, 71.84 ± 3.93/mm3 and 17.29 ± 3.54/mm3, respectively. The total number of OGCs and...Continue Reading

References

May 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H J GundersenA Vesterby
Mar 23, 2011·Human Reproduction·Ariel RevelEduardo Mitrani
Apr 2, 2013·Fertility and Sterility·Marie-Madeleine DolmansTine Greve
Feb 16, 2016·Nature Biotechnology·Hyun-Wook KangAnthony Atala
Apr 22, 2017·Critical Reviews in Oncology/hematology·Giovanni Codacci-PisanelliFedro A Peccatori
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Mar 28, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Benjamin Fisch, Ronit Abir
Feb 5, 2019·Acta Obstetricia Et Gynecologica Scandinavica·Mahmoud Salama, Teresa K Woodruff
Nov 26, 2019·Food Science & Nutrition·Zahra JafarinezhadSaied Karbalay-Doust

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