PMID: 9528689Apr 7, 1998Paper

A stereospecific phosphotriesterase in hen liver and brain

Chemico-biological Interactions
N Diaz-AlejoMiguel Angel Sogorb

Abstract

O-Hexyl, O-2,5-dichlorophenyl phosphoramidate (HDCP) is a chiral compound that induces delayed neuropathy in hens. This compound is hydrolyzed by a phosphotriesterase known as HDCPase in hen and rat plasma, liver and brain. We studied the stereospecificity of HDCPase in hen tissues and in human and rabbit plasma employing a chromatographic method for analysis and quantification of HDCP stereoisomers. Hen and human plasma HDCPases were not stereospecific. However, rabbit plasma showed a remarkable stereospecificity to S-(-)-HDCP. High levels of stereospecific HDCPase were found in the particulate fraction of hen liver, where S-(-)-HDCP is hydrolyzed faster than R-(+)-HDCP. However, in hen brain the stereospecificity was found in the soluble fraction, where R-(+)-HDCP is hydrolyzed faster than S-(-)-HDCP. It is concluded that liver particulate fraction must be the main tissue responsible for the HDCP stereospecific biotransformation in hens. In an oral administration, the steroisomer R-(+)-HDCP would survive after passing through the liver and would interact with acetylcholinesterase and neuropathy target esterase in the nervous system.

References

Aug 1, 1991·Biochemical Society Transactions·C H WalkerG Johnston
Nov 1, 1990·Neurotoxicology and Teratology·N Diaz-AlejoE Vilanova
Jun 1, 1993·Chemico-biological Interactions·M A SogorbN Díaz-Alejo
Jun 1, 1993·Chemico-biological Interactions·F C HoskinJ E Walker
Jun 1, 1993·Chemico-biological Interactions·K Kopec-SmythK B Ward
Feb 1, 1997·The Biochemical Journal·L RodrigoA Pla

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Citations

Aug 13, 2014·Archives of Toxicology·Antonio Monroy-NoyolaEugenio Vilanova

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