A steroid hormone affects sodium channel expression in Manduca central neurons

Cell and Tissue Research
J BörnerCarsten Duch

Abstract

Neuronal differentiation is characterized by stereotypical sequences of membrane channel and receptor acquisition. This is regulated by the coordinated interactions of a variety of developmental mechanisms, one of which is the control by steroid hormones. We have used the metamorphosis of the holometabolous insect, Manduca sexta, as a model to study effects of 20-hydroxyecdysone on the maturation of thoracic neuron membrane channel expression. To test for direct hormone action, neurons were dissociated into primary cell culture on the first day of pupal life. In situ hybridization demonstrated that the amount of expression of the acetylcholine receptor alpha subunit, MARA1, was not affected by 20-hydroxyecdysone. Immunocytochemistry with an antibody directed against the SP19 segment of voltage-gated sodium channels revealed no effect of 20-hydroxyecdysone treatment during the first 6 days in culture. SP19 sodium channel protein was evenly distributed along all neurites. In contrast, after 8 days in culture, 20-hydroxyecdysone increased the amount of SP19 protein expression and strongly affected its distribution in differentiating neurons. In the presence of 20-hydroxyecdysone, patches of high densities of SP19 sodium channel pr...Continue Reading

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Citations

Sep 8, 2010·Cellular and Molecular Life Sciences : CMLS·Floriano RodriguesChristian Klämbt
Jun 3, 2008·Der Anaesthesist·C C ClausenO Picker
Feb 9, 2013·The Journal of Experimental Biology·Julien Bacqué-CazenaveJean Paul Delbecque

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