PMID: 9547270Jun 20, 1998Paper

A stimulatory RNA associated with RecBCD enzyme

Nucleic Acids Research
S K AmundsenG R Smith

Abstract

RecBCD enzyme acts in the major pathway of homologous recombination of linear DNA in Escherichia coli. The enzyme unwinds DNA and is an ATP-dependent double-strand and single-strand exonuclease and a single-strand endonuclease; it acts at Chi recombination hotspots (5'-GCTGGTGG-3') to produce a recombinogenic single-stranded DNA 3'-end. We found that a small RNA with a unique sequence of approximately 24 nt was tightly bound to RecBCD enzyme and co-purified with it. When added to native enzyme this RNA, but not four others, increased DNA unwinding and Chi nicking activities of the enzyme. In seven similarly active enzyme preparations the molar ratio of RNA molecules to RecBCD enzyme molecules ranged from 0.2 to <0.008. These results suggest that, although this unique RNA is not an essential enzyme subunit, it has a biological role in stimulating RecBCD enzyme activity.

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A F Taylor, G R Smith
Jan 5, 1990·Journal of Molecular Biology·A F Taylor, G R Smith
Sep 25, 1987·Nucleic Acids Research·B Lankat-ButtgereitG Krupp
Apr 20, 1987·Journal of Molecular Biology·K C Cheng, G R Smith
Sep 20, 1985·Journal of Molecular Biology·A F Taylor, G R Smith
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S K AmundsenG R Smith
May 1, 1985·Cell·A S PonticelliG R Smith
Jun 4, 1971·Biochemical and Biophysical Research Communications·H U Blank, D Söll
Dec 5, 1984·Journal of Molecular Biology·K C Cheng, G R Smith
Nov 1, 1980·Cell·A Taylor, G R Smith
Oct 13, 1995·The Journal of Biological Chemistry·A F Taylor, G R Smith
Oct 13, 1995·The Journal of Biological Chemistry·A F Taylor, G R Smith
Jul 7, 1995·The Journal of Biological Chemistry·D A Dixon, S C Kowalczykowski

❮ Previous
Next ❯

Citations

Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Mark S Dillingham, Stephen C Kowalczykowski
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·S K AmundsenG R Smith
Oct 6, 2000·Annual Review of Microbiology·M Riley, M H Serres

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.