A stochastic model correctly predicts changes in budding yeast cell cycle dynamics upon periodic expression of CLN2

PloS One
Cihan OguzJohn J Tyson


In this study, we focus on a recent stochastic budding yeast cell cycle model. First, we estimate the model parameters using extensive data sets: phenotypes of 110 genetic strains, single cell statistics of wild type and cln3 strains. Optimization of stochastic model parameters is achieved by an automated algorithm we recently used for a deterministic cell cycle model. Next, in order to test the predictive ability of the stochastic model, we focus on a recent experimental study in which forced periodic expression of CLN2 cyclin (driven by MET3 promoter in cln3 background) has been used to synchronize budding yeast cell colonies. We demonstrate that the model correctly predicts the experimentally observed synchronization levels and cell cycle statistics of mother and daughter cells under various experimental conditions (numerical data that is not enforced in parameter optimization), in addition to correctly predicting the qualitative changes in size control due to forced CLN2 expression. Our model also generates a novel prediction: under frequent CLN2 expression pulses, G1 phase duration is bimodal among small-born cells. These cells originate from daughters with extended budded periods due to size control during the budded peri...Continue Reading


Mar 1, 1977·Experimental Cell Research·G C JohnstonL H Hartwell
Jul 1, 1977·Experimental Cell Research·P Nurse, P Thuriaux
Sep 13, 1996·Molecular & General Genetics : MGG·P HarrisP A Fantes
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·B Novak, John J Tyson
Mar 23, 2000·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·B Futcher
Jun 21, 2001·BioTechniques·P K DavisS F Dowdy
Jan 26, 2002·Molecular Biology of the Cell·F R CrossMartha Klovstad
Oct 17, 2003·Nature·Sina GhaemmaghamiJonathan S Weissman
Jan 25, 2007·Nature Reviews. Molecular Cell Biology·Joanna Bloom, F R Cross
Jan 30, 2008·Current Protocols in Cell Biology·J Jackman, P M O'Connor
Apr 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·G CharvinE D Siggia
Aug 10, 2010·BMC Bioinformatics·Suresh Kumar Poovathingal, Rudiyanto Gunawan
Jul 15, 2011·Methods in Molecular Biology·Arkadi ManukyanBrandt L Schneider
Aug 3, 2011·Yeast·K Wyatt McMahonBrandt L Schneider
May 12, 2012·Current Biology : CB·Jonathan J TurnerJan M Skotheim
Jun 16, 2012·Genetics·Erfei Bi, Hay-Oak Park
Apr 18, 2013·Molecular Systems Biology·Nurit AvrahamNaama Barkai


Oct 16, 2015·Journal of Theoretical Biology·Hafiz AhmedDenis Efimov
Oct 1, 2015·Scientific Reports·Hao Zhu, Yanlan Mao
May 14, 2016·Physical Biology·Neşe Aral, Alkan Kabakçıoğlu
Feb 24, 2017·Journal of the Royal Society, Interface·Michael B MayhewAlexander J Hartemink

Related Concepts

CLN2 protein, S cerevisiae
Cell Cycle
Saccharomyces cerevisiae
Stochastic Processes
Gene Expression Regulation, Fungal
Saccharomyces cerevisiae Proteins
Cell Volume

Related Feeds

Batten Disease

Batten Disease is a group of nervous system disorders known as neuronal ceroid lipofuscinosis. This feed focuses on neurobiological and neuropathological aspects of this disease.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Mark J Solomon
Protein Science : a Publication of the Protein Society
Jonathan S Weissman, Erin K O'Shea
© 2021 Meta ULC. All rights reserved