The mec-2 gene is required for the function of a set of six touch receptor neurons in the nematode Caenorhabditis elegans; mec-2 mutants, which are touch-insensitive, have touch cells that appear morphologically normal. Gene interaction studies suggest that mec-2 positively regulates the activity of the putative mechanosensory transduction channel (and the present paper), comprised in part of proteins encoded by the two degenerin genes mec-4 and mec-10 The central region of the mec-2 protein (MEC-2) is very similar to stomatin, an integral membrane protein (band 7.2b) in human red blood cells that is thought to regulate cation conductance. MEC-2-LacZ fusions are distributed along the touch receptor axons. This axonal distribution, which is mediated by the mec-2-specific amino terminus, is disrupted by mutations in mec-12, an alpha-tubulin gene needed for touch cell function. Our results indicate that MEC-2 links the mechanosensory channel and the microtubule cytoskeleton of the touch receptor neurons. Such linkage provides the basis for a mechanism of mechanosensation whereby microtubule displacement leads to channel opening.
Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins
The mec-4 gene is a member of a family of Caenorhabditis elegans genes that can mutate to induce neuronal degeneration
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mec-7 is a beta-tubulin gene required for the production of 15-protofilament microtubules in Caenorhabditis elegans
Anti-idiotypic antibodies that react with microtubule-associated proteins are present in the sera of rabbits immunized with synthetic peptides from tubulin's regulatory domain
A transmembrane domain of the putative channel subunit MEC-4 influences mechanotransduction and neurodegeneration in C. elegans
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Dehydrated hereditary stomatocytosis linked to gain-of-function mutations in mechanically activated PIEZO1 ion channels
The MEC-4 DEG/ENaC channel of Caenorhabditis elegans touch receptor neurons transduces mechanical signals
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Microtubule depolymerization in Caenorhabditis elegans touch receptor neurons reduces gene expression through a p38 MAPK pathway
Sequence and transmembrane topology of MEC-4, an ion channel subunit required for mechanotransduction in Caenorhabditis elegans
MEC-2 and MEC-6 in the Caenorhabditis elegans sensory mechanotransduction complex: auxiliary subunits that enable channel activity
Protein interaction surface of the POU transcription factor UNC-86 selectively used in touch neurons
Regulation of ASIC channels by a stomatin/STOML3 complex located in a mobile vesicle pool in sensory neurons
Identification, localization, and functional implications of the microdomain-forming stomatin family in the ciliated protozoan Paramecium tetraurelia
Functional characterization and modified rescue of novel AE1 mutation R730C associated with overhydrated cation leak stomatocytosis
TRPC6 channels and their binding partners in podocytes: role in glomerular filtration and pathophysiology
Podocin, a raft-associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin
NPHS2 mutations in late-onset focal segmental glomerulosclerosis: R229Q is a common disease-associated allele
Schistosoma mansoni Stomatin like protein-2 is located in the tegument and induces partial protection against challenge infection
Importance of non-selective cation channel TRPV4 interaction with cytoskeleton and their reciprocal regulations in cultured cells
Two novel DEG/ENaC channel subunits expressed in glia are needed for nose-touch sensitivity in Caenorhabditis elegans
The DEG/ENaC protein MEC-10 regulates the transduction channel complex in Caenorhabditis elegans touch receptor neurons
mec-15 encodes an F-box protein required for touch receptor neuron mechanosensation, synapse formation and development
Structural characterization and mutational assessment of podocin - a novel drug target to nephrotic syndrome - an in silico approach
Chromatin immunoprecipitation (ChIP) scanning identifies primary glucocorticoid receptor target genes
Role of the ubiquitin-proteasome system in nervous system function and disease: using C. elegans as a dissecting tool
Stomatin-domain protein interactions with acid-sensing ion channels modulate nociceptor mechanosensitivity
Association of stomatin with lipid-protein complexes in the plasma membrane and the endocytic compartment
Stomatin is mis-trafficked in the erythrocytes of overhydrated hereditary stomatocytosis, and is absent from normal primitive yolk sac-derived erythrocytes
Plasma membrane targeting of podocin through the classical exocytic pathway: effect of NPHS2 mutations
TRPV1-tubulin complex: involvement of membrane tubulin in the regulation of chemotherapy-induced peripheral neuropathy
The hereditary stomatocytoses: genetic disorders of the red cell membrane permeability to monovalent cations
unc-8, a DEG/ENaC family member, encodes a subunit of a candidate mechanically gated channel that modulates C. elegans locomotion
Extracellular proteins organize the mechanosensory channel complex in C. elegans touch receptor neurons
In vivo imaging of C. elegans mechanosensory neurons demonstrates a specific role for the MEC-4 channel in the process of gentle touch sensation
MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch receptor neurons through its stomatin-like domain
The multipurpose 15-protofilament microtubules in C. elegans have specific roles in mechanosensation
GFP is a selective non-linear optical sensor of electrophysiological processes in Caenorhabditis elegans
Membrane raft actin deficiency and altered Ca2+-induced vesiculation in stomatin-deficient overhydrated hereditary stomatocytosis
Genomewide search for dehydrated hereditary stomatocytosis (hereditary xerocytosis): mapping of locus to chromosome 16 (16q23-qter)
The hereditary stomatocytoses and allied disorders: congenital disorders of erythrocyte membrane permeability to Na and K
Identification of a stomatin orthologue in vacuoles induced in human erythrocytes by malaria parasites. A role for microbial raft proteins in apicomplexan vacuole biogenesis
NPHS2, encoding the glomerular protein podocin, is mutated in autosomal recessive steroid-resistant nephrotic syndrome
Taxonomic affinities and evolutionary history of the Early Pleistocene hominids of Java: dentognathic evidence
Temperature-sensitive mutant of the Caenorhabditis elegans neurotoxic MEC-4(d) DEG/ENaC channel identifies a site required for trafficking or surface maintenance
Sulfated signal from ASJ sensory neurons modulates stomatin-dependent coordination in Caenorhabditis elegans
Presynaptic MAST kinase controls opposing postsynaptic responses to convey stimulus valence in Caenorhabditis elegans
Identification and characterization of human SLP-2, a novel homologue of stomatin (band 7.2b) present in erythrocytes and other tissues
Stomatin-like protein 2 deficiency in T cells is associated with altered mitochondrial respiration and defective CD4+ T cell responses
Insights into the molecular mechanisms underlying the inhibition of acid-sensing ion channel 3 gating by stomatin
A family showing recessively inherited multisystem pathology with aberrant splicing of the erythrocyte Band 7.2b ('stomatin') gene
The Candida albicans stress response gene Stomatin-Like Protein 3 is implicated in ROS-induced apoptotic-like death of yeast phase cells
Opposing effects of podocin on the gating of podocyte TRPC6 channels evoked by membrane stretch or diacylglycerol
Life with Bacterial Mechanosensitive Channels, from Discovery to Physiology to Pharmacological Target
Activation of the Caenorhabditis elegans Degenerin Channel by Shear Stress Requires the MEC-10 Subunit
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