PMID: 2497729Apr 1, 1989Paper

A stopped-flow kinetic study of soluble methane mono-oxygenase from Methylococcus capsulatus (Bath)

The Biochemical Journal
J Green, H Dalton

Abstract

1. The roles of the three protein components of soluble methane mono-oxygenase were investigated by the use of rapid-reaction techniques. The transfer of electrons through the enzyme complex from NADH to methane/O2 was also investigated. 2. Electron transfer from protein C, the reductase component, to protein A, the hydroxylase component, was demonstrated. Protein C was shown to undergo a three-electron--one-electron catalytic cycle. The interaction of protein C with NADH was investigated. Reduction of protein C was shown to be rapid, and a charge-transfer interaction between reduced FAD and NAD+ was observed; this intermediate was also found in static titration experiments. Thus the binding of NADH, the reduction of protein C and the intramolecular transfer of electrons through protein C were shown to be much more rapid than the turnover rate of methane mono-oxygenase. 3. The rate of transfer of electrons from protein C to protein A was shown to be lower than the reduction of protein C but higher than the turnover rate of methane mono-oxygenase. Association of the proteins was not rate-limiting. The amount of protein A present in the system had a small effect on the rate of reduction of protein C, indicating some co-operativit...Continue Reading

Citations

Aug 27, 2015·Biochemistry·Justin F AchesonBrian G Fox
Nov 11, 2016·Applied Biochemistry and Biotechnology·Thomas HeineDirk Tischler
Dec 1, 1992·European Journal of Biochemistry·D D Smith, H Dalton
Jan 12, 2013·Applied Microbiology and Biotechnology·Kianoush Khosravi-DaraniKenji Tanaka
Mar 20, 2010·FEMS Microbiology Reviews·Jeremy D SemrauSukhwan Yoon
Nov 14, 2003·The Journal of Biological Chemistry·Jessica L Blazyk, Stephen J Lippard
Apr 7, 2004·Applied and Environmental Microbiology·Valeria CafaroAlberto Di Donato
Oct 20, 1995·The Journal of Biological Chemistry·Y LiuJ D Lipscomb
Nov 7, 1996·Chemical Reviews·Bradley J. Wallar, John D. Lipscomb
Dec 8, 2005·Journal of the American Chemical Society·Jessica L BlazykStephen J Lippard

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