PMID: 19531894Jun 18, 2009

A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: possibility for automatic screening of beta-thalassemia trait

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Supada KhonyoungKate Grudpan

Abstract

Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline solution. A portion of red cells was merged on-line with a hypotonic saline solution. They were mixed while flowing into the detector and were stopped for a short period for continuously monitoring the change of turbidity based on a transmission signal. In this work, a possible application of the system for screening of beta-thalassemia trait is demonstrated. Descriptions of instrumentation development and parameter optimization are presented. The system offers advantages over the conventional batch-wise OFT in terms of automation, precision, analysis time and sample volume.

References

Aug 1, 1988·Biophysical Journal·H A MassaldiH C Mel
Jun 1, 1986·Journal of Cellular Physiology·H A MassaldiH C Mel
May 1, 1983·British Journal of Industrial Medicine·K FukumotoS Horiguchi
May 1, 1982·British Journal of Industrial Medicine·I KaraiS Horiguchi
Jan 1, 1981·International Archives of Occupational and Environmental Health·I KaraiS Horiguchi
Aug 1, 1981·Journal of Medical Genetics·C KattamisS Pootrakul
Apr 23, 2003·Early Human Development·Maria L G BautistaRaul A Wapnir
Sep 10, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Z K LiG S Khush
Aug 25, 2004·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·S SirichotiyakulT Tongsong
Jun 28, 2005·American Journal of Hematology·Jason ChowBarbara J Bain
Feb 12, 2008·International Journal of Clinical Practice·P B McBethA W Kirkpatrick
Jul 17, 2010·International Journal of Laboratory Hematology·M MamtaniH Kulkarni

Citations

Related Concepts

Erythrocytes
Osmotic Fragility Test
Spectrophotometry
Flow Injection Analysis
Thalassemia Minor
Beta Thalassemia
Erythrocytes
Osmotic Fragility Test
Biologic Development
Analysis

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.

Related Papers

International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics
S SirichotiyakulT Tongsong
International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics
S SirichotiyakulT Tongsong
Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Sarawut SomnamShoji Motomizu
© 2021 Meta ULC. All rights reserved