PMID: 9525759Apr 3, 1998Paper

A strain device imposing dynamic and uniform equi-biaxial strain to cultured cells

Annals of Biomedical Engineering
M SotoudehShu Chien

Abstract

The objective of this study is to design a new apparatus to allow the control of the magnitude and frequency of dynamic stretch applied uniformly to cells cultured on a silicon elastic membrane. The apparatus is designed to produce equi-biaxial dynamic stretches with area changes ranging from 0% to 55% and frequencies ranging from 0 to 2 Hz. Homogeneous finite strain analysis using triangles of markers was performed to compute the symmetric two-dimensional Lagrangian strain tensor on the membrane. Measurements of strain in both static and dynamic conditions showed that the shear component of the strain tensor (Erc) was near zero, and that there was no significant difference between radial (Err) and circumferential (Ecc) components, indicating the attainment of equi-biaxial strain. Bovine aortic endothelial cells were transiently transfected with a chimeric construct in which the luciferase reporter is driven by TPA-responsive elements (TRE). The transfected cells cultured on the membrane were stretched. The luciferase activity increased significantly only when the cells were stretched by 15% or more in area. Cells in different locations of the membrane showed similar induction of luciferase activities, confirming that strain is...Continue Reading

Citations

May 25, 2010·Biomechanics and Modeling in Mechanobiology·P N WattonY Ventikos
Feb 3, 2009·Annals of Biomedical Engineering·Adele M DoyleTabassum Ahsan
Mar 3, 2010·Annals of Biomedical Engineering·Lawrence HuangBrian P Helmke
May 24, 2006·Biomedical Microdevices·N ScuorV Sergo
Dec 28, 1999·Journal of Biomechanics·T D Brown
Apr 5, 2007·Nature Protocols·Patrizia CamellitiAndrew D McCulloch
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Jul 10, 2010·Sports Medicine, Arthroscopy, Rehabilitation, Therapy & Technology : SMARTT·James Hc Wang, Bin Li
Oct 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Roland KaunasShu Chien
Jan 6, 2009·Journal of Biomechanics·Yu Ching YungDavid J Mooney
May 14, 2008·Biochemical and Biophysical Research Communications·Jason H HagaTammy M Seasholtz
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Jun 26, 2012·Biotechnology and Bioengineering·Elissa K LeonardElizabeth J Orwin
Jul 27, 2006·Journal of Biomechanics·Jason H HagaShu Chien
Oct 31, 2015·Journal of Biomechanics·Candice Sears, Roland Kaunas
Sep 13, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Rui-Hai ZhouJohn Y-J Shyy
Nov 14, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Shu Chien
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Apr 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Mohammad SotoudehJohn Y-J Shyy
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Sep 28, 2017·Bioengineering·Ismaeel MuhamedVenkat Maruthamuthu
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Apr 12, 2019·Frontiers in Bioengineering and Biotechnology·Oliver FriedrichBoris Martinac
Oct 21, 2011·American Journal of Physiology. Cell Physiology·Naama ShohamAmit Gefen

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