PMID: 9637683Jun 24, 1998Paper

A stress response pathway from the endoplasmic reticulum to the nucleus requires a novel bifunctional protein kinase/endoribonuclease (Ire1p) in mammalian cells

Genes & Development
W TirasophonR J Kaufman

Abstract

Eukaryotes respond to the presence of unfolded protein in the endoplasmic reticulum (ER) by up-regulating the transcription of genes encoding ER protein chaperones, such as BiP. We have isolated a novel human cDNA encoding a homolog to Saccharomyces cerevisiae Ire1p, a proximal sensor for this signal transduction pathway in yeast. The gene product hIre1p is a type 1 transmembrane protein containing a cytoplasmic domain that is highly conserved to the yeast counterpart having a Ser/Thr protein kinase domain and a domain homologous to RNase L. However, the luminal domain has extensively diverged from the yeast gene product. hIre1p expressed in mammalian cells displayed intrinsic autophosphorylation activity and an endoribonuclease activity that cleaved the 5' splice site of yeast HAC1 mRNA, a substrate for the endoribonuclease activity of yeast Ire1p. Overexpressed hIre1p was localized to the ER with particular concentration around the nuclear envelope and some colocalization with the nuclear pore complex. Expression of Ire1p mRNA was autoregulated through a process that required a functional hIre1p kinase activity. Finally, overexpression of wild-type hIre1p constitutively activated a reporter gene under transcriptional control ...Continue Reading

References

Jan 2, 1992·Nature·M J Gething, J Sambrook
Dec 1, 1986·The Journal of Cell Biology·E ResendezA S Lee
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J ShenJ Subjeck
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S C ChangA S Lee
Jan 1, 1995·Methods in Enzymology·H PatersonD Robertson
Feb 1, 1994·International Journal of Radiation Oncology, Biology, Physics·A C KoongA J Giaccia
Jan 1, 1997·Methods in Molecular Biology·R J Kaufman
Dec 31, 1997·The Journal of Biological Chemistry·J C Morris, K Mensa-Wilmot
Feb 28, 1998·Current Biology : CB·R E Chapman, P Walter

❮ Previous
Next ❯

Citations

May 27, 2009·Cellular and Molecular Life Sciences : CMLS·Ann-Hwee Lee, Laurie H Glimcher
Mar 11, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hideaki KanetoYoshimitsu Yamasaki
Jan 12, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Peter ScrivenLynda Wyld
Mar 27, 2013·Seminars in Immunopathology·Takao Iwawaki, Daisuke Oikawa
Apr 11, 2013·Seminars in Immunopathology·Sang Won Park, Umut Ozcan
Mar 16, 2012·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Gong-Lu LiuYong-Bo Zhao
Apr 11, 2009·Apoptosis : an International Journal on Programmed Cell Death·Vanya I Rasheva, Pedro M Domingos
Jun 1, 2011·Journal of Ocular Biology, Diseases, and Informatics·Sarah X ZhangJoshua J Wang
Jan 25, 2012·Cell Stress & Chaperones·Heather F BurnettRui Ray Lu
Mar 25, 2011·Environmental Health and Preventive Medicine·David A AremuShunsuke Meshitsuka
Dec 31, 2003·Trends in Immunology·Jennifer N GassJoseph W Brewer
Sep 15, 2004·Journal of Chemical Neuroanatomy·Yanjun Ma, Linda M Hendershot
Nov 11, 2003·Life Sciences·Masayuki Kaneko, Yasuyuki Nomura
Jul 6, 2004·Neurochemistry International·Kayoko OonoMasaya Tohyama
May 3, 2003·Biochemical and Biophysical Research Communications·Rai KneeMarek Michalak
May 22, 2002·Biochimica Et Biophysica Acta·Seung-Jae NohWon-Il Chung
Feb 17, 2001·Current Opinion in Structural Biology·E ChevetJ J Bergeron
Jan 25, 2005·Nature Cell Biology·Shinichi KondoKazunori Imaizumi
Oct 23, 2012·Nature Chemical Biology·Likun WangDustin J Maly
Dec 22, 2004·Nature Immunology·Joseph W Brewer, Linda M Hendershot
Dec 2, 2004·Nature Reviews. Cancer·Yanjun Ma, Linda M Hendershot
Nov 26, 2008·Oncogene·R B HamanakaJ A Diehl
Apr 14, 2009·Médecine sciences : M/S·Marion Bouchecareilh, Eric Chevet
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Takao IwawakiKenji Kohno
Feb 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Akio TsuruKenji Kohno
Oct 24, 2009·The Journal of Biological Chemistry·Ming LiuMichael A Weiss
Feb 10, 2011·The Journal of Biological Chemistry·Kori VolkmannJohn B Patterson
Mar 25, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M Díaz-GuerraM Esteban
Mar 3, 2007·Antioxidants & Redox Signaling·Atsushi SaitoKazunori Imaizumi
Sep 22, 2006·Antioxidants & Redox Signaling·Agnes GörlachThomas Kietzmann
Nov 6, 2007·Antioxidants & Redox Signaling·Jyoti D Malhotra, Randal J Kaufman
Sep 27, 2007·Antioxidants & Redox Signaling·Sonya G FonsecaFumihiko Urano
Sep 25, 2007·Antioxidants & Redox Signaling·Daniel Bailey, Peter O'Hare
Nov 6, 2007·Antioxidants & Redox Signaling·Hiderou Yoshida

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.