A stringent requirement for Plk1 T210 phosphorylation during K-fiber assembly and chromosome congression.

Chromosoma
Catherine Randall PaschalPrasad V Jallepalli

Abstract

Polo-like kinase 1 (Plk1) is an essential mitotic regulator and undergoes periodic phosphorylation on threonine 210, a conserved residue in the kinase's activation loop. While phosphate-mimicking alterations of T210 stimulate Plk1's kinase activity in vitro, their effects on cell cycle regulation in vivo remain controversial. Using gene targeting, we replaced the native PLK1 locus in human cells with either PLK1 (T210A) or PLK1 (T210D) in both dominant and recessive settings. In contrast to previous reports, PLK1 (T210D) did not accelerate cells prematurely into mitosis, nor could it fulfill the kinase's essential role in chromosome congression. The latter was traced to an unexpected defect in Plk1-dependent phosphorylation of BubR1, a key mediator of stable kinetochore-microtubule attachment. Using chemical genetics to bypass this defect, we found that Plk1(T210D) is nonetheless able to induce equatorial RhoA zones and cleavage furrows during mitotic exit. Collectively, our data indicate that K-fibers are sensitive to even subtle perturbations in T210 phosphorylation and caution against relying on Plk1(T210D) as an in vivo surrogate for the natively activated kinase.

References

Nov 24, 1999·Molecular and Cellular Biology·Y W QianJ L Maller
Sep 12, 2000·Nature Cell Biology·V A SmitsR H Medema
May 8, 2002·The Journal of Biological Chemistry·Olaf KelmErich A Nigg
Jul 31, 2002·The Journal of Biological Chemistry·Paula B DemingWilliam K Kaufmann
Sep 19, 2002·The Journal of Biological Chemistry·Young-Joo JangRaymond L Erikson
Jan 14, 2003·Nature Cell Biology·Mark JackmanJonathon Pines
Jul 11, 2003·Developmental Cell·Tianhua ZhouRaymond L Erikson
Jan 22, 2004·The Journal of Cell Biology·Catherine Lindon, Jonathon Pines
Sep 8, 2004·Molecular Cell·Marcel A T M van VugtRené H Medema
Jan 11, 2005·Oncogene·Drew M LoweryMichael B Yaffe
Sep 1, 2005·The Journal of Cell Biology·Oi Kwan Wong, Guowei Fang
Sep 3, 2005·Nature Cell Biology·Maura PapiPrasad V Jallepalli
Sep 15, 2005·The Journal of Biological Chemistry·Wei ZhangRuth J Muschel
Nov 2, 2005·The Journal of Cell Biology·Tomoya YamaguchiMasaki Inagaki
Mar 2, 2006·Molecular and Cellular Biology·Xiaoqi LiuRaymond L Erikson
Jul 25, 2006·Current Biology : CB·Michiya NishinoLi-Yuan Yu-Lee
Feb 13, 2007·Current Biology : CB·Péter LénártJan-Michael Peters
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mark E BurkardPrasad V Jallepalli
Mar 23, 2007·The Journal of Biological Chemistry·Shigeru MatsumuraEisuke Nishida
Apr 2, 2008·The Journal of Cell Biology·Akiko SekiGuowei Fang
May 15, 2008·Developmental Cell·Mark PetronczkiJan-Michael Peters
Jul 11, 2008·Nature·Libor MacůrekRené H Medema
Mar 24, 2009·Nature Reviews. Molecular Cell Biology·Vincent Archambault, David M Glover
Mar 25, 2009·Molecular and Cellular Biology·Kazuhiro KishiMichael B Yaffe
May 29, 2009·Methods in Molecular Biology·Eli BerdougoPrasad V Jallepalli
Jul 27, 2010·Current Biology : CB·Jadranka LoncarekAlexey Khodjakov

❮ Previous
Next ❯

Citations

Oct 19, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Robert F Lera, Mark E Burkard
Jan 27, 2015·Oncogene·V ArchambaultD Kachaner
Jun 17, 2020·Cell Cycle·João BarbosaClaudio Sunkel
Jan 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Michal FeldmanDan Levy
Dec 29, 2017·Molecular Biology of the Cell·Erica G ColicinoHeidi Hehnly
Apr 1, 2014·Molecular & Cellular Oncology·Stephane Schmucker, Izabela Sumara
Jul 3, 2018·Frontiers in Physiology·Martin HennenbergChristian Gratzke
Dec 7, 2020·Cytoskeleton·Joseph Y OngJorge Z Torres
Feb 16, 2020·Cell·Yongchao DouUNKNOWN Clinical Proteomic Tumor Analysis Consortium

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Molecular and Cellular Biology
Kazuhiro KishiMichael B Yaffe
Nature Reviews. Molecular Cell Biology
Vincent Archambault, David M Glover
Trends in Biochemical Sciences
Frank Eckerdt, James L Maller
© 2021 Meta ULC. All rights reserved