A structural analysis of M protein in coronavirus assembly and morphology

Journal of Structural Biology
Benjamin W NeumanMichael J Buchmeier

Abstract

The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.

References

Jan 1, 1979·Archives of Virology·H A Davies, M R Macnaughton
Apr 1, 1992·The Journal of General Virology·R W RuigrokR H Wade
Aug 1, 1986·Virus Research·B G Hogue, D A Brian
Oct 1, 1995·The Journal of Cell Biology·D J OpsteltenP J Rottier
Jul 15, 1994·Structure·Y HarpazC Chothia
Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Nov 23, 1987·Physical Review Letters·O Y Zhong-can, W Helfrich
Aug 5, 1999·The Biochemical Journal·M RamjeesinghC E Bear
Dec 22, 1999·Journal of Structural Biology·S J LudtkeW Chiu
May 9, 2000·Journal of Virology·C A de HaanP J Rottier
Nov 7, 2000·Biochimica Et Biophysica Acta·J F Nagle, S Tristram-Nagle
Dec 13, 2002·Journal of Virology·Eric J SnijderWilly J M Spaan
Mar 29, 2003·Journal of Molecular Biology·Chun TangMichael F Summers
May 19, 2004·Biochemistry·Qiulong HuangEdward T Olejniczak
Nov 24, 2004·Ultramicroscopy·Alex Stewart, Nikolaus Grigorieff
May 14, 2005·Journal of Structural Biology·Christian SulowayBridget Carragher
Jul 26, 2005·Nature Structural & Molecular Biology·Jana StichtHans-Georg Kräusslich
Jun 17, 2006·Science·Aurélie A V AlbertiniRob W H Ruigrok
Jul 18, 2006·Nature Structural & Molecular Biology·Daniel R BeniacTim F Booth
Sep 22, 2006·Journal of Virology·Heike SchützeJohn Ziebuhr
Dec 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Audray HarrisAlasdair C Steven
Jan 11, 2008·Journal of Virology·Lisa A LopezBrenda G Hogue
Jan 11, 2008·Journal of Virology·Joseph A BoscarinoThomas M Gallagher
Mar 28, 2008·Journal of Virology·Benjamin W NeumanPeter Kuhn
Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qinghong ZengRaoul J de Groot
Dec 10, 2008·Cell Host & Microbe·Lars-Anders CarlsonHans-Georg Kräusslich
Jan 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Montserrat BárcenaBerend Jan Bosch

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Citations

Jul 19, 2013·Journal of the Royal Society, Interface·David SchleyBenjamin W Neuman
Apr 10, 2015·Viruses·Makoto Ujike, Fumihiro Taguchi
Feb 24, 2016·Frontiers in Plant Science·Olivia C DemurtasRosella Franconi
Sep 3, 2011·Current Opinion in Virology·Chi-yu Fu, Johnson E Johnson
Aug 28, 2018·Journal of Cellular Physiology·Somayeh ShokriFatemeh Farshadpour
Sep 6, 2018·PloS One·Antonina NaskalskaKrzysztof Pyrc
May 28, 2019·Virology Journal·Dewald Schoeman, Burtram C Fielding
Jul 19, 2019·Journal of Virology·Antonina NaskalskaKrzysztof Pyrc
Apr 3, 2020·Vaccines·Jinyong ZhangQuanming Zou
May 6, 2020·Viruses·Maddalena DiluccaAthanasia Pavlopoulou
Apr 2, 2020·ELife·Yinon M Bar-OnRon Milo
Apr 30, 2020·Journal of the Chinese Medical Association : JCMA·Tai-Jay ChangChin-Tien Wang
Jun 13, 2020·Expert Review of Respiratory Medicine·Arafat HussainAnant Mohan
Jul 4, 2020·Interdisciplinary Sciences, Computational Life Sciences·Abbas KhanDong-Qing Wei
May 15, 2020·Annals of Surgery·Camille L StewartLaleh G Melstrom
Jul 15, 2020·The Journal of Biological Chemistry·Ella HartenianBritt A Glaunsinger
Jul 25, 2016·Diseases·Yvonne Xinyi LimDing Xiang Liu
Mar 16, 2019·Frontiers in Veterinary Science·Juan Carlos Mora-DíazLuis Gabriel Giménez-Lirola
Jul 1, 2020·Turkish Journal of Biology = Türk Biyoloji Dergisi·Israt Jahan, Ahmet Onay
Aug 7, 2020·American Journal of Physiology. Endocrinology and Metabolism·Emile LevySchohraya Spahis
Sep 26, 2020·Frontiers in Immunology·Sana O AlturkiElias K Haddad
Apr 2, 2020·Journal of Clinical Microbiology·Wanbing LiuShangen Zheng
May 8, 2020·Biomedicines·Kenneth Lundstrom
Jul 1, 2020·Infectious Diseases·Mohamed M HassanSofia B Mohamed
Jul 2, 2020·International Journal of Molecular Sciences·Alejandro LlanesRicardo Lleonart
Sep 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Simona PiccolellaSeverina Pacifico
Jun 26, 2020·Clinical Microbiology Reviews·Kuldeep DhamaAlfonso J Rodriguez-Morales
Jul 29, 2020·Frontiers in Cell and Developmental Biology·Jin Zhou, Jie Huang
Jun 18, 2020·PeerJ·Müşerref Duygu Saçar Demirci, Aysun Adan
Mar 24, 2020·Future Virology·Entedar A J Alsaadi, Ian M Jones
Jul 7, 2020·Frontiers in Neurology·Jianhan HuangLiangxue Zhou
Jul 28, 2020·Cellular and Molecular Life Sciences : CMLS·Rajanish GiriVladimir N Uversky
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Hasan UludağAzita Haddadi

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Methods Mentioned

BETA
electron microscopy
transfection
protein assay
electrophoresis
environmental stress

Software Mentioned

EMAN
classalign2
ctfit
imod
M LONG
LONG
SPIDER
COMPACT
M COMPACT

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