PMID: 6397469Jan 1, 1984Paper

A structural analysis of the role of the nuclear matrix and DNA loops in the organization of the nucleus and chromosome

Journal of Cell Science. Supplement
K J Pienta, D S Coffey

Abstract

The interphase nucleus is characterized by a nuclear matrix structure that forms a residual scaffolding composed of approximately 10% of the total nuclear proteins. The nuclear matrix contains residual elements of the pore-complex and lamina, the nucleolus, and an intranuclear fibrous network that provides the basic shape and structure of the nucleus. In the interphase nucleus this nuclear matrix has been reported to be a central element in the organization of DNA loop domains and to contain fixed sites for DNA replication and transcription. In this study, we have analysed the role of the nuclear matrix and the DNA loop domains in the organization and structure of the number 4 human chromosome. A model is proposed that closely approximates the observed structural dimensions of this chromosome. The model is composed of 30 nm diameter filaments formed from a solenoid of six nucleosomes per turn. This 30 nm solenoid filament is organized as loops of DNA each containing approximately 60 000 base-pairs; each loop is anchored at its base to the nuclear matrix. A radial loop model containing 18 of these loops per turn forms a new unit of chromosome structure termed the miniband. Approximately 106 of these minibands are arranged along ...Continue Reading

Citations

Jan 1, 1993·The Prostate·K J Pienta, J E Lehr
Mar 1, 1987·Plant Molecular Biology·J S ZlatanovaB S Stanchev
Sep 1, 1989·Chromosoma·W S WardD S Coffey
May 4, 1989·Molecular and Cellular Biochemistry·N I ValkovC P Krachmarov
Jun 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·R MartinG Wanner
Feb 4, 2009·European Biophysics Journal : EBJ·Isaac GállegoJoan-Ramon Daban
Apr 10, 2003·Chromosoma·Wolfgang Hennig
Apr 15, 2005·Histochemistry and Cell Biology·Sabine M GörischKarsten Rippe
Oct 20, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Juan Manuel CaravacaJoan-Ramon Daban
Dec 1, 1989·Mechanisms of Ageing and Development·A JaberaboansariK T Wheeler
Jan 1, 1995·Advances in Enzyme Regulation·D J FernandesC V Catapano
Jan 1, 1991·Molecular Aspects of Medicine·A T VaughanA M Milner
Aug 1, 1989·Molecular Immunology·M MonestierL Böhm
Jan 1, 1988·Pharmacology & Therapeutics·V A Bohr, P C Hanawalt
Jul 15, 1996·International Journal of Radiation Oncology, Biology, Physics·R S MalyapaJ L Roti Roti
May 1, 1989·Mutation Research·A Macieira-Coelho, F Puvion-Dutilleul
Jul 19, 2002·Advances in Enzyme Regulation·Ronald Berezney
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S I DworetzkyG S Stein
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·S K KeeseeY J Wu
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·J A NickersonS Penman
Sep 15, 2009·Molecular Human Reproduction·W Steven Ward
May 19, 2011·Nucleic Acids Research·Pavithra Lakshminarasimhan ChavaliSreenivas Chavali
Nov 21, 2012·The Journal of Cell Biology·Kumiko SamejimaWilliam C Earnshaw
Dec 16, 1998·The Journal of Cell Biology·H MaR Berezney
Nov 1, 1987·Journal of Electron Microscopy Technique·S SekiT Oda
Nov 7, 1987·Journal of Theoretical Biology·M Takahashi
Sep 6, 2005·Advances in Enzyme Regulation·Ronald BerezneyRaj Acharya
Dec 26, 2012·Current Opinion in Genetics & Development·Yin C Lin, Cornelis Murre
Aug 30, 2008·Biochimica Et Biophysica Acta·Malte WachsmuthKarsten Rippe
Oct 27, 2009·Journal of Molecular Biology·Peter R Cook
Jun 5, 2004·BMC Biology·Tony Chih Yuan ChuangSabine Mai
Feb 22, 2011·Toxicon : Official Journal of the International Society on Toxinology·Otilia AntalAgnes Vehovszky
Aug 30, 2016·Cell Biology International·Maria A Kuznetsova, Eugene V Sheval

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.