Abstract
Arginine 115 in the subsite F of human lysozyme (peptidoglycan N-acetylmuramoylhydrolase, EC 3.2.1.17) was replaced with lysine, histidine, glutamine or glutamine acid by site-directed mutagenesis. The conversions which conserve positive charge, Arg115 to Lys or His (at acidic pH), have little affected on either the kinetic parameters for Micrococcus lysodeikticus cells or the activity against glycol chitin, nor on the cleavage patterns of hexa(N-acetylglucosamine) [(GlcNAc)6] and penta(N-acetylglucosamine) [(GlcNAc)5]. On the other hand, the conversions which cause loss of the positive charge, Arg115 to His (neutral and alkaline pH), Gln or Glu, not only reduced the activity against glycol chitin but also changed the cleavage patterns for (GlcNAc)6 and (GlcNAc)5. These results suggest that Arg115 is structurally required not for the specific hydrogen bonding interaction with a sugar residue but for the positively charged character in the construction of subsite F in human lysozyme.
References
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 16, 1979·Nature·P J ArtymiukM J Sternberg
Feb 25, 1987·Biochimica Et Biophysica Acta·M MurakiH Tanaka
Apr 1, 1988·Protein Engineering·M MurakiH Tanaka
Oct 1, 1987·Journal of Biochemistry·I KumagaiK Miura
Jan 1, 1986·Gene·Y JigamiH Tanaka
Nov 5, 1987·Biochimica Et Biophysica Acta·M MurakiH Tanaka
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Apr 18, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·P Jollès
Apr 18, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C C BlakeV R Sarma
Aug 1, 1969·Science·D M Chipman, N Sharon
Sep 29, 1970·Biochemistry·J J Pollock, N Sharon
Aug 27, 1968·Biochimica Et Biophysica Acta·J P LocquetP Jollès
Mar 1, 1969·Archives of Biochemistry and Biophysics·R M ParryK M Shahani
May 22, 1965·Nature·C C BlakeV R Sarma
Sep 1, 1984·Molecular and Cellular Biochemistry·P Jollès, J Jollès
Jul 1, 1983·Journal of Biochemistry·T FukamizoK Hayashi
Feb 28, 1984·Biochemistry·S J Smith-GillH A Scheraga
Sep 1, 1982·Journal of Biochemistry·T FukamizoK Hayashi
Aug 1, 1981·Journal of Biochemistry·A MasakiT Imoto
Nov 15, 1981·Journal of Molecular Biology·P J Artymiuk, C C Blake
Citations
Oct 2, 2001·Bioscience, Biotechnology, and Biochemistry·T FukamizoK M Vårum
May 2, 2003·Bioscience, Biotechnology, and Biochemistry·Gen ToshimaTakao Torikata
Mar 8, 2008·Bioscience, Biotechnology, and Biochemistry·Shunsuke KawamuraTakao Torikata
Apr 29, 2010·The Journal of Chemical Physics·Koji UmezawaJunichi Higo
Nov 11, 2016·PloS One·Shalini KalraJai K Kaushik
Aug 7, 1990·Biochemistry·C Redfield, C M Dobson
Sep 29, 1992·Biochemistry·M MurakiY Jigami
Dec 5, 1994·FEBS Letters·M MurakiK Harata
Sep 22, 2017·PLoS Pathogens·Stephanie A Ragland, Alison K Criss
Jan 13, 2000·Biochemistry·M MurakiK I Sato