A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM Technology

Journal of Analytical Methods in Chemistry
Yibing WangPing Wang

Abstract

Studying cell microstructures and their behaviors under living conditions has been a challenging subject in microbiology. In this work, in situ liquid chamber TEM was used to study structures of Escherichia coli cells in aqueous solutions at a nanometer-scale resolution. Most of the cells remained intact under electron beam irradiation, and nanoscale structures were observed during the TEM imaging. The analysis revealed structures of pili surrounding the E. coli cells; the movements of the pili in the liquid were also observed during the in situ tests. This technology also allowed the observation of features of the nucleoid in the E. coli cells. Overall, in situ TEM can be applied as a valuable tool to study real-time microscopic structures and processes in microbial cells residing in native aqueous solutions.

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Citations

Apr 5, 2017·The Review of Scientific Instruments·Sofia M JohanssonJan B C Pettersson
Apr 8, 2020·International Journal of Nanomedicine·David J BannerTolou Shokuhfar
Dec 14, 2018·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Kun HeReza Shahbazian-Yassar
Nov 13, 2018·Micron : the International Research and Review Journal for Microscopy·Trevor H MoserJames E Evans

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Methods Mentioned

BETA
chips
transmission electron microscopy
fluorescence microscopy
imaging techniques
electron diffraction

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