A structurally deviant member of the Euplotes raikovi pheromone family: Er-23

The Journal of Eukaryotic Microbiology
Giuseppe Graziano DiP Luporini

Abstract

Pheromones of Euplotes raikovi form a homologous family of proteins with 37- to 40-amino acid residues, including six cysteines that form three strictly conserved disulfide bridges. The determination of the primary structure of the pheromone Er-23, which was isolated from cells derived from natural populations of E. raikovi that secrete the other known pheromones, has now revealed a novel structure type. The polypeptide chain of this pheromone contains 51 residues, 10 of which are cysteines presumably involved in the formation of five disulfide bridges, and lacks a carboxyl-terminal tail following the last cysteine of the sequence. The elongation of the Er-23 molecule is presumed to result from multiple events of gene duplication starting from an ancestral motif Xxx(2-4)-Cys-Xxx(5-7)-Cys.

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Citations

Jun 28, 2016·European Journal of Protistology·Pierangelo LuporiniAdriana Vallesi
Jun 8, 2018·Biomolecular NMR Assignments·David P BowlesThomas J Magliery

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Methods Mentioned

BETA
nuclear magnetic resonance
PCR
gel
ion-exchange chromatography
electrophoresis

Software Mentioned

COMPLEX

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