A structure-guided mutation in the major capsid protein retargets BK polyomavirus

PLoS Pathogens
Ursula NeuThilo Stehle

Abstract

Viruses within a family often vary in their cellular tropism and pathogenicity. In many cases, these variations are due to viruses switching their specificity from one cell surface receptor to another. The structural requirements that underlie such receptor switching are not well understood especially for carbohydrate-binding viruses, as methods capable of structure-specificity studies are only relatively recently being developed for carbohydrates. We have characterized the receptor specificity, structure and infectivity of the human polyomavirus BKPyV, the causative agent of polyomavirus-associated nephropathy, and uncover a molecular switch for binding different carbohydrate receptors. We show that the b-series gangliosides GD3, GD2, GD1b and GT1b all can serve as receptors for BKPyV. The crystal structure of the BKPyV capsid protein VP1 in complex with GD3 reveals contacts with two sialic acid moieties in the receptor, providing a basis for the observed specificity. Comparison with the structure of simian virus 40 (SV40) VP1 bound to ganglioside GM1 identifies the amino acid at position 68 as a determinant of specificity. Mutation of this residue from lysine in BKPyV to serine in SV40 switches the receptor specificity of BKP...Continue Reading

References

Jul 2, 2014·Current Opinion in Virology·Samantha D O'HaraRobert L Garcea
Jun 25, 2014·Virus Research·Bethany A O'HaraWalter J Atwood
Nov 3, 2014·Annual Review of Virology·Luisa J Ströh, Thilo Stehle
Feb 11, 2014·Current Opinion in Chemical Biology·Angelina S PalmaYan Liu
Aug 1, 2014·Journal of Neurovirology·Melissa S MaginnisWalter J Atwood
Oct 27, 2015·Medical Microbiology and Immunology·Danijela KaralicTanja Jovanovic
Jun 4, 2014·Virology·Stephanie J BouleyAisling S Dugan
Oct 17, 2015·PLoS Pathogens·John YouThomas L Benjamin
Oct 27, 2016·Viruses·Heidi BarthSamira Fafi-Kremer
Jul 18, 2015·Cancers·Raquel Burger-Calderon, Jennifer Webster-Cyriaque
Nov 4, 2017·Viruses·Francois HelleGilles Duverlie
Nov 21, 2017·MSphere·Saumya MaruAron E Lukacher
May 9, 2014·Journal of Virology·Thilo Stehle, Zaigham Mahmood Khan
Mar 5, 2015·MBio·Jennifer Stencel-BaerenwaldTerence S Dermody
Mar 13, 2019·Clinical Transplantation·Hans H HirschAST Infectious Diseases Community of Practice
Jan 4, 2020·Journal of Virology·Lynda HandalaFrancois Helle
Aug 1, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·San-Yuan ChenDeching Chang
Mar 5, 2020·Reviews in Medical Virology·Jason T BlackardBenjamin L Laskin
Oct 11, 2017·Current Clinical Microbiology Reports·Colleen L MayberryMelissa S Maginnis

Citations

Nov 28, 1991·Nature·R C LiddingtonS C Harrison
Jan 1, 1990·Archives of Virology·L SinibaldiN Orsi
May 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A BediR J Jones
Feb 1, 1994·Protein Science : a Publication of the Protein Society·E A MerrittW G Hol
Dec 1, 1993·Journal of Medical Virology·T ShinoharaK Nagashima
Oct 24, 1998·American Journal of Physical Anthropology·E A MuchmoreA Varki
Aug 28, 2003·Current Opinion in Microbiology·Karsten Hueffer, Colin R Parrish
Aug 28, 2003·The EMBO Journal·Billy TsaiTom A Rapoport
Oct 3, 2003·The Lancet Infectious Diseases·Hans H Hirsch, Jürg Steiger
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·Collaborative Computational Project, Number 4
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Jul 12, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Hans H Hirsch
Sep 6, 2005·Journal of Virology·Sylvia Eash, Walter J Atwood
Jan 18, 2006·Journal of Virology·Jonathan A LowMichael J Imperiale
Jan 5, 2007·Nucleic Acids Research·M FrankC-W von der Lieth
Apr 11, 2007·Physiology·Kenneth J Linton
Aug 19, 2007·Journal of Virology·Aisling S DuganWalter J Atwood
Sep 15, 2007·Journal of Virology·Maria A Campanero-RhodesTen Feizi
Mar 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ursula NeuThilo Stehle
Oct 11, 2008·Journal of Lipid Research·Robert K YuMakoto Yanagisawa
Oct 17, 2008·Physiological Reviews·Susan F Steinberg
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Jun 12, 2010·The Journal of Biological Chemistry·Steven J Gamblin, John J Skehel
Jul 30, 2010·Journal of Virology·Mengding Qian, Billy Tsai
Mar 12, 2013·Nature Reviews. Microbiology·James A DeCaprio, Robert L Garcea
Jun 14, 1999·Angewandte Chemie·Moriz Mayer, Bernd Meyer

Related Concepts

Right Upper Arm Structure
GT1b synthase
Casein allergenic extract
Molecular Dynamics
Study
Flow Cytometry
Biochemical Pathway
Amidophosphoribosyltransferase
Positioning Attribute
Crystal - Body Material

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