A structure of the complex between concanavalin A and methyl-3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside reveals two binding modes.

The Journal of Biological Chemistry
R LorisJ Bouckaert

Abstract

The structure of concanavalin A in complex with the trimannoside methyl-3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside has been determined in a novel space group. In three of the four subunits of the concanavalin A tetramer, the interactions between the protein and the bound saccharide are essentially identical to those reported previously by other authors (Naismith, J. H., and Field, R. A. (1996) J. Biol. Chem. 271, 972-976). In the fourth subunit, however, the alpha1-->3 linkage has a different conformation, resulting in a different part of the alpha1-->3-linked mannose interacting with essentially the same surface of the protein. Furthermore, significant differences are observed in the quaternary associations of the subunits compared with the saccharide-free structures and other carbohydrate complexes, suggesting that the concanavalin A tetramer is a rather flexible entity.

References

Mar 29, 1985·Biochemical and Biophysical Research Communications·F BrewerS H Koenig
Dec 19, 1972·Biochemistry·K D Hardman, C F Ainsworth
Oct 27, 1995·The Journal of Biological Chemistry·F CassetA Imberty
Dec 1, 1994·Nature Structural Biology·Y BourneC Cambillau
Jan 12, 1996·The Journal of Biological Chemistry·J H Naismith, R A Field
Jul 5, 1996·The Journal of Biological Chemistry·J BouckaertR Loris
Jul 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·G J Kleywegt
Jul 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·J BouckaertL Wyns
Jan 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·S J HarropJ Yariv
Nov 1, 1995·Acta Crystallographica. Section D, Biological Crystallography·A J KalbJ Yariv

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Citations

Aug 31, 2010·Journal of the American Society for Mass Spectrometry·Amr El-HawietJohn S Klassen
Jan 20, 2004·Journal of Molecular Biology·Remy LorisJulie Bouckaert
Apr 18, 1998·Biochimica Et Biophysica Acta·R LorisL Wyns
Nov 5, 1997·Current Opinion in Structural Biology·A Imberty
Dec 5, 2008·Journal of the American Chemical Society·Renuka KadirvelrajRobert J Woods
Aug 15, 2006·The Journal of Physical Chemistry. a·Sarah M Tschampel, Robert J Woods
Sep 7, 2006·Nature Reviews. Molecular Cell Biology·Martin Chaplin
Mar 31, 2012·Physical Chemistry Chemical Physics : PCCP·Madhurima Jana, Sanjoy Bandyopadhyay
Jun 14, 2012·The Journal of Biological Chemistry·Raquel Guimarães BenevidesAnne Imberty
Oct 24, 2012·Glycobiology·Diego F GautoMarcelo A Martí
Jun 22, 2000·Biochemical and Biophysical Research Communications·D JainD M Salunke
Nov 26, 2008·Bioorganic & Medicinal Chemistry·Kazusa NishiyamaSusumu Kobayashi
Jan 24, 2007·Physical Chemistry Chemical Physics : PCCP·Zheng Li, Themis Lazaridis
Jun 13, 2019·Scientific Reports·Fabrizio FierroMercedes Alfonso-Prieto
Oct 2, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Hara JangSeung Jae Lee
Dec 15, 2010·Chembiochem : a European Journal of Chemical Biology·Kuo-Ting HuangNicolas Winssinger
Jan 13, 2019·International Journal of Molecular Sciences·Annick BarrePierre Rougé
Jun 3, 2009·The Journal of Physical Chemistry. B·Diego F GautoMarcelo A Martí
Feb 14, 2006·The Journal of Physical Chemistry. B·Zheng Li, Themis Lazaridis
May 21, 2019·International Journal of Biological Macromolecules·Benildo Sousa CavadaKyria Santiago Nascimento
Jun 8, 2021·Journal of the American Chemical Society·Benjamin E PartridgeChad A Mirkin
May 6, 2017·Journal of Medicinal Chemistry·Francesca SpyrakisGlen E Kellogg
Dec 6, 2001·Journal of the American Chemical Society·C ClarkeG J Boons
Jul 21, 2006·The Journal of Physical Chemistry. B·Zheng Li, Themis Lazaridis
Oct 8, 2021·ACS Chemical Biology·Laura L Kiessling, Roger C Diehl
May 26, 2007·The Journal of Physical Chemistry. B·Santiago Di LellaJuan C Díaz Ricci

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