A structured chitosan-based platform for biomolecule attachment to solid surfaces: application to DNA microarray preparation

Bioconjugate Chemistry
Clarissa ConsolandiGianluca De Bellis

Abstract

A structured chemical platform based on chitosan, an amine-rich polysaccharide, is presented as an alternative chemistry to functionalize solid support (in this case, glass slides) for grafting biomolecules. This approach has been adopted for generating arrays using amino-modified oligonucleotides with two different lengths (25-mer and 70-mer) for different purposes. Results using these chitosan-activated surfaces indicate high oligonucleotide loading capacity, good availability to hybridization against targets, and effectiveness in enzyme-mediated single nucleotide polymorphism (SNP) detection procedures by DNA polymerase and DNA ligase enzymes with low background. Universal arrays have been prepared and extensively used with excellent results in different applications. The chitosan-treated surfaces were also evaluated for their performance in a gene expression experiment.

References

Feb 3, 1998·Nature Biotechnology·G Ramsay
Jan 23, 1999·Nature Genetics·R J LipshutzD J Lockhart
Jul 4, 2001·Nucleic Acids Research·K LindroosA C Syvänen
Mar 28, 2002·Nucleic Acids Research·Ramesh RamakrishnanAbhijit Mazumder

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Citations

Apr 6, 2013·Chemical Reviews·Wipa SugintaAlbert Schulte
Jun 8, 2012·BMC Microbiology·Marco CandelaPatrizia Brigidi
Feb 1, 2013·The Journal of Nutrition, Health & Aging·S RampelliP Brigidi
Oct 22, 2009·Biotechnology Journal·Dalip SethiKailash Chand Gupta
Sep 27, 2006·IEEE Transactions on Nanobioscience·Marco SevergniniSergio Cerutti
Feb 27, 2008·Analytical Biochemistry·Clarissa ConsolandiGianluca De Bellis
Nov 14, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Enzo SpisniAntonio Strillacci
Feb 8, 2008·Bioconjugate Chemistry·María-José BañulsAngel Maquieira

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