A study of biomarkers in cervical carcinoma and clinical correlation of the novel biomarker MN

Gynecologic Oncology
C A BrewerEric J Stanbridge

Abstract

The MN protein is a newly described biomarker found to be overexpressed in most cervical carcinomas. This study was an effort to evaluate the prognostic importance of tumor MN expression, HPV status, and the presence of other biomarkers in cervical cancers. Tumor DNA and protein for study were extracted from archived frozen tissue. Tumor tissues and controls were evaluated by Western blot analysis for MN, intestinal alkaline phosphatase (IAP), c-myc, and p53 protein overexpression. Immunohistochemistry was performed for MN quantification and the study of expression patterns in histologic subtypes of cervical cancer. HPV data were obtained by PCR amplification of extracted DNA using consensus and type-specific primers. Clinical data were obtained from the patients' records and from the cancer registry. Clinical and molecular data were correlated by chi2, Fisher's exact test, and logistic regression. The results demonstrate that IAP is not overexpressed in clinical specimens of cervical carcinoma, although in somatic cell hybrid experiments, overexpression of IAP correlates with the malignant state. None of 47 tumors, including those which were HPV negative, overexpressed p53. c-myc protein overexpression occurred in 11 of 52 tum...Continue Reading

Citations

Mar 22, 2002·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·C D GolijowF N Dulout
Oct 10, 2014·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M Pérez-SayánsA García-García
Nov 15, 2008·Pathology, Research and Practice·S RasheedT Guenther
Jun 11, 2008·Pathology, Research and Practice·Shahnawaz RasheedThomas Guenther
Oct 11, 2003·Alimentary Pharmacology & Therapeutics·M JuhászM P A Ebert
Mar 16, 2012·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Mario Pérez-SayánsAbel García-García
Apr 11, 2012·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Mario Pérez-SayánsAbel García-García
Oct 13, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Hidehiro Kon-noAtsushi Ochiai
May 30, 2006·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Ruth A JonathanJohannes H A M Kaanders
Dec 3, 2014·Biochimica Et Biophysica Acta·Enrico Pierluigi SpugniniStefano Fais
Aug 21, 2013·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M Pérez-SayánsA García-García
Nov 27, 2010·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·G SergeantB Topal
May 31, 2013·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Saskia E RademakersJohannes H Kaanders
Apr 18, 2002·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S M F BrennaK J Syrjanen
Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·R KlaesM von Knebel Doeberitz
Apr 28, 2005·International Journal of Cancer. Journal International Du Cancer·Magdalena KowalewskaJanusz A Siedlecki
Nov 26, 1999·British Journal of Cancer·H UemuraE Oosterwijk
Jun 6, 2008·The Journal of Veterinary Medical Science·Yi-Fan JenMing-Huang Chang
Nov 1, 2002·The American Journal of Surgical Pathology·Rüdiger KlaesMagnus von Knebel Doeberitz
Aug 16, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S K ChiaA L Harris
Nov 3, 2021·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Andreina FernandesRene Pareja

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