A study of concentration changes of Protoporphyrin IX and Coproporphyrin III in mixed samples mimicking conditions inside cancer cells for Photodynamic Therapy

PloS One
Rainer LandesMichael Friebe

Abstract

Photodynamic Therapy (PDT) using Aminolevulinic acid (ALA) could be an effective and minimally invasively applicable way to treat many different types of tumors without radiation and large incisions by just applying a light pulse. However the PDT process is difficult to observe, control and optimize and the dynamical relationships between the variables involved in the process is complex and still hardly understood. One of the main variables affecting the outcome of the process is the determination of the interval of time between ALA inoculation and starting of light delivery. This interval, better known as drug-light interval, should ensure that enough Protoporphyrin IX (PPIX) is located in the vicinity of functional structures inside the cells for the greatest damage during the PDT procedure. One route to better estimate this time interval would be by predicting PPIX from the dynamical changes of its precursors. For that purpose, in this work a novel optical setup (OS) is proposed for differentiating fluorescence emitted by Coproporphyrin III (CPIII) and PPIX itself in samples composed of mixed solutions. The OS is tested using samples with different concentrations in mixed solutions of PPIX and the precursor CPIII as well as ...Continue Reading

Associated Clinical Trials

References

Jan 19, 2000·Clinical Biochemistry·J T HindmarshD C Greenway
Jan 6, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jonathan S Dysart, Michael S Patterson
Feb 6, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Reinhard SailerHerbert Schneckenburger
Feb 5, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Pawel SwietachAlzbeta Hulikova
Jun 13, 2015·European Journal of Dermatology : EJD·Colin MortonUNKNOWN European Dermatology Forum
Oct 27, 2016·Cancers·Diana K Cohen, Peter K Lee

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Citations

Jun 15, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Lilia Coronato Courrol, Marcelo Afonso Vallim

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Methods Mentioned

BETA
Fluorescence

Software Mentioned

Labview

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