PMID: 1201144Nov 1, 1975Paper

A study of different methods for the assay of lipoprotein lipase activity in human adipose tissue

Atherosclerosis
B PerssonB Larsson

Abstract

A method for the assay of lipoprotein lipase activity (LPLA) in heparin eluates of needle biopsies of adipose tissue is presented. A serum activated phosphatidestabilized emulsion of labelled triolein has been used as substrate. This method and a previously described method using heparin eluates as enzyme source and a commercial triglyceride emulsion, Ediol, as substrate, showed a high degree of correlation (correlation coefficient = 0.94) when parallel determinations were performed on biopsies from 16 subjects. Further, the Ediol method similarly correlated well with a method for LPLA assay, previously described by Nilsson-Ehle and Belfrage, on acetone-ether extracts of adipose tissue (correlation coefficient = 0.88; 19 subjects).

References

Aug 20, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·P Nilsson-Ehle
Mar 1, 1970·European Journal of Clinical Investigation·E Shafrir, Y Biale
Sep 12, 1967·Biochimica Et Biophysica Acta·R G MorganB Borgström
Mar 1, 1964·Proceedings of the Society for Experimental Biology and Medicine·D V DATTA, H S WIGGINS

Citations

Jan 27, 1978·Biochimica Et Biophysica Acta·H Lithell, J Boberg
May 21, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·M R TaskinenH Hilden
Aug 1, 1987·Baillière's Clinical Endocrinology and Metabolism·M R Taskinen, T Kuusi
Oct 1, 1977·Scandinavian Journal of Clinical and Laboratory Investigation·H Lithell, J Boberg
Mar 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J D BagdadeM R Taskinen

Related Concepts

Fat Pad
Bos indicus
Clinical Enzyme Tests (Procedure)
Lipidemias
Lipoprotein LIPASE
Triglycerides
Triolein
Tritium

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