PMID: 14927794Mar 1, 1952Paper

A study of fixation for electron microscopy

The Journal of Experimental Medicine
G E PALADE

Abstract

Osmium tetroxide fixation of tissue blocks, as usually effected, is preceded by an acidification of the tissue. This acidification is probably responsible for morphological alterations which are notably disturbing in electron microscopy. The acidification and the resulting morphological alterations cannot be prevented by homogenizing the tissue directly in OsO(4) solutions or by adding enzyme inhibitors (fluoride, iodoscetamide) to the fixative. Fixation experiments with buffered OsO(4) solutions have shown that the appearance of the fixed cells is conditioned by the pH of the fixative. The quality of fixation can be materially improved by buffering the OsO(4) solutions at pH 7.3-7.5, The acetate-veronal buffer appeared to be the most favorable of the buffers tested, Because of these findings, 1 per cent OsO(4) buffered at pH 7.3-7.5 with acetate-veronal buffer is recommended as an appropriate fixative for electron microscopy.

References

Jun 1, 1950·Biochimica Et Biophysica Acta·L W LABAWR W G WYCKOFF
Nov 3, 1950·Science·J HILLIER, M E GETTNER
Jun 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·H LATTA, J F HARTMANN

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Citations

May 1, 1957·The American Journal of Anatomy·R L SCHULTZD C PEASE
Sep 1, 1958·The American Journal of Anatomy·R G HIBBS
Jan 1, 1959·The American Journal of Anatomy·B L SCOTT, D C PEASE
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Jul 1, 1960·The American Journal of Anatomy·W T DEMPSTER
May 1, 1961·The American Journal of Anatomy·S DONAHUE, G D PAPPAS
Sep 1, 1961·The American Journal of Anatomy·S S HAN
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May 1, 1979·Annals of Neurology·S DiMauroL Z Stern
Nov 1, 1952·The Anatomical Record·R A ROBINSON, M L WATSON
Nov 1, 1952·The Anatomical Record·G E PALADE
Jul 1, 1953·The Anatomical Record·S L PALAY, S L WISSIG
Jul 1, 1953·The Anatomical Record·B G MONROE
Oct 1, 1953·The Anatomical Record·F N LOW
Jan 1, 1954·The Anatomical Record·R H SWIGART, D J KANE
Apr 1, 1955·The Anatomical Record·D C PEASE
Jul 1, 1956·The Anatomical Record·J S MARK
Jan 1, 1957·The Anatomical Record·F N LOW, M M SAMPAIO
Apr 1, 1958·The Anatomical Record·C M GARNER, D DUNCAN

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