A study of new Arabidopsis chloroplast RNA editing mutants reveals general features of editing factors and their target sites.

The Plant Cell
Kamel HammaniIan Small

Abstract

RNA editing in higher plant organelles results in the conversion of specific cytidine residues to uridine residues in RNA. The recognition of a specific target C site by the editing machinery involves trans-acting factors that bind to the RNA upstream of the C to be edited. In the last few years, analysis of mutants affected in chloroplast biogenesis has identified several pentatricopeptide repeat (PPR) proteins from the PLS subfamily that are essential for the editing of particular RNA transcripts. We selected other genes from the same subfamily and used a reverse genetics approach to identify six new chloroplast editing factors in Arabidopsis thaliana (OTP80, OTP81, OTP82, OTP84, OTP85, and OTP86). These six factors account for nine editing sites not previously assigned to an editing factor and, together with the nine PPR editing proteins previously described, explain more than half of the 34 editing events in Arabidopsis chloroplasts. OTP80, OTP81, OTP85, and OTP86 target only one editing site each, OTP82 two sites, and OTP84 three sites in different transcripts. An analysis of the target sites requiring the five editing factors involved in editing of multiple sites (CRR22, CRR28, CLB19, OTP82, and OTP84) suggests that editi...Continue Reading

References

Mar 25, 1991·Nucleic Acids Research·K EdwardsC Thompson
Jan 1, 1996·Annual Review of Neuroscience·L Simpson, R B Emeson
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·T ShikanaiA Yokota
Jun 4, 1999·The Journal of Heredity·R M MulliganM T Shanahan
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·P Giegé, A Brennicke
Feb 9, 2000·Trends in Biochemical Sciences·I D Small, N Peeters
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Jul 13, 2000·Journal of Molecular Biology·O EmanuelssonG von Heijne
Nov 26, 2002·Molecular and Cellular Biology·Anne-Laure Chateigner-Boutin, Maureen R Hanson
May 9, 2003·Nucleic Acids Research·Anne-Laure Chateigner-Boutin, Maureen R Hanson
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Mihoko HashimotoToshiharu Shikanai
Dec 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya MiyamotoMasahiro Sugiura
Aug 6, 2004·Nucleic Acids Research·Mizuki TakenakaAxel Brennicke
Sep 18, 2004·BMC Bioinformatics·Michael P Cummings, Daniel S Myers
Mar 19, 2005·The Plant Journal : for Cell and Molecular Biology·Carla E HegemanMaureen R Hanson
Feb 9, 2006·Cellular and Molecular Life Sciences : CMLS·T Shikanai
Sep 20, 2006·Nucleic Acids Research·Sigrid D AuweterFrédéric H-T Allain
Oct 4, 2006·The Journal of Biological Chemistry·Kenji OkudaToshiharu Shikanai
Nov 14, 2006·Plant Physiology·Monika W MurchaJames Whelan
Jan 5, 2007·Annual Review of Plant Biology·Toshiharu Shikanai
May 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kenji OkudaToshiharu Shikanai
Jun 15, 2007·Genetics·Stéphane BentolilaMaureen R Hanson
Aug 21, 2007·FEBS Letters·Véronique SaloneClaire Lurin
Mar 8, 2008·Mitochondrion·Mizuki TakenakaAxel Brennicke
Mar 18, 2008·Molecular Biology and Evolution·Nicholas O'TooleIan Small
Jul 4, 2008·The Journal of Biological Chemistry·Daniil VerbitskiyMizuki Takenaka
Jul 29, 2008·The Plant Journal : for Cell and Molecular Biology·Anne-Laure Chateigner-BoutinPatricia León
Oct 16, 2008·The Journal of Biological Chemistry·Lianwei PengToshiharu Shikanai
Nov 14, 2008·Trends in Plant Science·Christian Schmitz-Linneweber, Ian Small
Nov 28, 2008·The Plant Journal : for Cell and Molecular Biology·Chris CarrieJames Whelan

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Citations

Apr 29, 2010·Journal of Molecular Evolution·Daniel B Sloan, Douglas R Taylor
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sota FujiiIan D Small
May 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Stephane BentolilaMaureen R Hanson
Mar 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mizuki TakenakaAxel Brennicke
Sep 15, 2012·The Journal of Biological Chemistry·Daniil VerbitskiyMizuki Takenaka
Sep 20, 2011·Journal of Experimental Botany·Tomohiro KakizakiTakehito Inaba
Jan 26, 2011·Molecular Biology and Evolution·Michael L Hayes, R Michael Mulligan
Dec 1, 2011·Nucleic Acids Research·Keiko KobayashiTakahiro Nakamura
Dec 6, 2011·Nucleic Acids Research·Hannes Ruwe, Christian Schmitz-Linneweber
Jun 3, 2011·Plant Physiology·Aleel K Grennan
Oct 26, 2011·Biochemistry. Biokhimii︠a︡·B Castandet, A Araya
Nov 28, 2013·Annual Review of Genetics·Mizuki TakenakaAxel Brennicke
Mar 29, 2014·Plant Physiology·Christopher R JohnJulian M Hibberd
Mar 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Tao SunStéphane Bentolila
Jan 30, 2014·Annual Review of Plant Biology·Alice Barkan, Ian Small
Mar 3, 2010·Annual Review of Plant Biology·David B SternMaureen R Hanson
Sep 18, 2010·Cellular and Molecular Life Sciences : CMLS·Jessica Jacobs, Ulrich Kück
Oct 13, 2010·Cellular and Molecular Life Sciences : CMLS·Volker Knoop
Mar 26, 2013·FEBS Letters·Hannes RuweDavid B Stern

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