A study of the B-A transition in DNA by gel electrophoresis

Journal of Biomolecular Structure & Dynamics
L S Shlyakhtenko

Abstract

A procedure is developed for studying the B-A transition in DNA using gel electrophoresis. The starting point has been the idea that the junction between the A and B sections, which appear within the transition interval would increase the mobility of the DNA molecules. Indeed, the mobility of DNA in a gel is shown to increase in the middle of the B-A transition due to the formation of the largest possible number of boundaries between the B and A forms. The middle of the B-A transition in supercoiled DNA appears to be shifted against the middle of the transition in open circular (as well as linear) DNA by about 1.3% towards lower ethanol concentrations under the influence of the superhelical stress.

References

Sep 21, 1978·Nature·E SelsingD R Kearns
Aug 25, 1974·Journal of Molecular Biology·V I IvanovA K Schyolkina
Jun 1, 1984·Journal of Biomolecular Structure & Dynamics·I A Mikhailenko, L S Shlyakhtenko
Feb 11, 1980·Nucleic Acids Research·V N PotamanL S Shlyachtenko

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Citations

Jan 1, 1988·Molecular Aspects of Medicine·N C OlsonC E McCall
Nov 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·P PregnolatoV Barzanti
Feb 10, 2016·Annals of Human Biology·Pankaj ShrivastavaVeena Ben Trivedi
Aug 1, 1985·Journal of Biomolecular Structure & Dynamics·V I IvanovE E Minyat

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