A study of the binding between polymers and peptides, using affinity capillary electrophoresis, applied to polymeric drug delivery systems.

Electrophoresis
Frédéric ProgentDanielle Ferrier

Abstract

We have investigated the potential of affinity capillary electrophoresis (ACE) to evaluate binding constants between an anionic polydispersed polymer and four peptides. Nonlinear regression and three current linearization methods, the y-reciprocal, the x-reciprocal and the double-reciprocal, were employed for the estimation of the binding constants. The x-reciprocal and the double-reciprocal plots indicated the presence of two portions of straight lines for angiopeptin, triptorelin and the thyrotropin releasing hormone (TRH), and therefore the probable existence of a second-order interaction which causes the deviation from the 1:1 model. Peptide 1 exhibited a unique binding constant of 2.4 x 10(6)M(-1). In contrast, angiopeptin, triptorelin and TRH exhibited a K(1) of 4.0 x 10(6), 5.3 x 10(6) and 20.2 x 10(6)M(-1), respectively, and a K(2) of 0.4 x 10(6), 0.5 x 10(6) and 1.4 x 10(6)M(-1), respectively. The origin of the high scattering of the data points was further investigated. Neither the viscosity, nor the adsorption of the peptides to the capillary wall appeared to be the determining factor of data scattering. Finally, a possible adsorption of the polymer leading to the electroosmotic flow instability was supposed.

References

Oct 7, 1994·Journal of Chromatography. a·N H Heegaard
Jul 1, 1995·Bioconjugate Chemistry·W R Gombotz, D K Pettit
Sep 13, 1996·Journal of Chromatography. a·N H HeegaardK L Larsen
Feb 10, 1998·Electrophoresis·G RippelJ Frank
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·N H HeegaardN A Guzman
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·J Kawaoka, F A Gomez
Oct 12, 2000·Biophysical Journal·F Sha, F M Chen
Feb 24, 2001·Electrophoresis·R M Guijt-van DuijnE Baltussen
May 31, 2001·Electrophoresis·K L Rundlett, D W Armstrong

❮ Previous
Next ❯

Citations

Sep 29, 2005·Analytical and Bioanalytical Chemistry·Dinora ChinchillaFrank A Gomez
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C BertucciV Andrisano
Feb 5, 2003·Current Opinion in Biotechnology·Chiara Galbusera, David D Y Chen
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Carolina SimóAlberto Gallardo
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Cinzia TozziGianfranco Giraudi
Dec 17, 2009·Journal of Pharmaceutical Sciences·Jean-Baptiste GuilbaudYaroslav Z Khimyak
Jan 30, 2010·Journal of Pharmaceutical Sciences·Jean-Baptiste GuilbaudYaroslav Z Khimyak
Jun 12, 2009·Electrophoresis·Niels H H Heegaard
Jun 23, 2007·Biomedical Chromatography : BMC·Suresh C V BabuYoung Sook Yoo
Dec 30, 2009·Electrophoresis·Chunxia Jiang, Daniel W Armstrong
Apr 23, 2005·Journal of Chromatography. a·Hervé CottetAlejandro Cifuentes
Aug 26, 2016·Analytical and Bioanalytical Chemistry·Pavel DubskýMartin Ansorge
May 16, 2009·Analytical Chemistry·Mark D GoldbergFrank A Gomez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.