A study of the Candida albicans cell wall proteome

Proteomics
Luis CastilloRafael Sentandreu

Abstract

Considering the importance of proteins in the structure and function of the cell wall of Candida albicans, we analyzed the cell wall subproteome of this important human pathogen by LC coupled to MS (LC-MS) using different protein extraction procedures. The analyzed samples included material extracted by hydrogen fluoride-pyridine (HF-pyridine), and whole SDS-extracted cell walls. The use of this latter innovative procedure gave similar data as compared to the analysis of HF-pyridine extracted proteins. A total of 21 cell wall proteins predicted to contain a signal peptide were identified, together with a high content of potentially glycosylated Ser/Thr residues, and the presence of a GPI motif in 19 of them. We also identified 66 "atypical" cell wall proteins that lack the above-mentioned characteristics. After tryptic removal of the most accessible proteins in the cell wall, several of the same expected GPI proteins and the most commonly found "atypical" wall proteins were identified. This result suggests that proteins are located not only at the cell wall surface, but are embedded within the cell wall itself. These results, which include new identified cell wall proteins, and comparison of proteins in blastospore and mycelial...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·F Sherman
Jul 1, 1987·Archives of Microbiology·E ValentínR Sentandreu
Aug 22, 1969·Biochemical and Biophysical Research Communications·M V Elorza, R Sentandreu
Oct 1, 1966·Journal of Bacteriology·J R Trevithick, R L Metzenberg
Jul 1, 1994·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·I SaraisG P Rocco
Jan 1, 1997·Nucleic Acids Research·H W MewesF Pfeiffer
Jul 8, 2003·Yeast·Piet W J De GrootFrans M Klis
Apr 1, 1964·Journal of Bacteriology·P GERHARDT, J A JUDGE
May 7, 2004·The Journal of Biological Chemistry·Donald C SheppardJohn E Edwards
Aug 23, 2005·Medical Mycology·Eric S Jacobson, Reiko Ikeda
Oct 26, 2005·Proteomics·M Graciela PucciarelliFrancisco García-del Portillo
Dec 17, 2005·Trends in Microbiology·César NombelaW LaJean Chaffin
Jan 21, 2006·FEMS Yeast Research·José Ruiz-HerreraRafael Sentandreu
Feb 4, 2006·Fungal Genetics and Biology : FG & B·Luis CastilloRafael Sentandreu
Feb 25, 2006·Yeast·Frans M KlisPiet W J De Groot
Mar 21, 2006·International Journal for Parasitology·Jaap J Van HellemondAloysius G M Tielens

❮ Previous
Next ❯

Citations

Dec 8, 2010·Journal of Proteome Research·Lucia MonteolivaConcha Gil
Aug 16, 2011·Eukaryotic Cell·Anita BoisraméMathias L Richard
Jul 14, 2009·Genome Biology·Richard J Bennett
Aug 25, 2011·Future Microbiology·Frans M KlisStanley Brul
Aug 19, 2009·Proteomics·Rafael Prados-RosalesAntonio Di Pietro
May 14, 2011·International Journal of Medical Microbiology : IJMM·Olaf KniemeyerAxel A Brakhage
Feb 24, 2010·Journal of Proteomics·María Rosa InsenserConcha Gil
Jun 12, 2012·Proteomics·Vital VialásW LaJean Chaffin
Nov 3, 2010·Mass Spectrometry Reviews·Ganesh Kumar AgrawalRandeep Rakwal
Oct 5, 2013·Journal of Chemotherapy·Elisabetta VavalaLetizia Angiolella
Nov 7, 2009·FEMS Yeast Research·José Ruiz-Herrera, Lucila Ortiz-Castellanos
Jun 14, 2012·FEMS Yeast Research·Patrícia de Sousa LimaCélia Maria de Almeida Soares
Mar 29, 2011·FEMS Yeast Research·Leslie LaforetEulogio Valentín-Gomez
Dec 8, 2015·Applied and Environmental Microbiology·Xinping XuXiaorong Lin
Jul 4, 2019·World Journal of Microbiology & Biotechnology·Yamily Y Burgos-CanulIgnacio Islas-Flores
Sep 7, 2019·Current Protein & Peptide Science·Verónica PlazaLuis Castillo
Jul 18, 2020·Microorganisms·Dorota SatalaAndrzej Kozik
Dec 2, 2020·International Journal of Molecular Sciences·Renata TeparićVladimir Mrša
Feb 13, 2021·Journal of Fungi·Mateja LozančićVladimir Mrša
Oct 28, 2020·International Journal of Molecular Sciences·Dorota SatalaAndrzej Kozik
May 18, 2021·Future Microbiology·Relber A GonçalesPaulo Sr Coelho
May 18, 2017·Microbiology Spectrum·Neil A R GowCarol A Munro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.