PMID: 2484456Dec 1, 1989Paper

A study of the differentiation inducibility of cultured cells by environmental chemical substances

Nihon eiseigaku zasshi. Japanese journal of hygiene
M Kamakura

Abstract

The author conducted an experimental study of the cell differentiation inducibility of some environmental chemical substances which currently draw attention because of their influences on the human body, using an azaguanine-resistant PCC4 cell line, a clone derived from mouse teratocarcinoma OTT 6050. Cells in monolayer culture or suspension culture were exposed to the test substances and the survival and morphological changes of the cells were determined. It was extremely rare that embryonal carcinoma cells exposed and cultured in a monolayer showed differentiation in the control group, with the rate of both epithelial cell-like and fibroblast-like morphological changes being less than 1%. Cells cultured in normal medium following exposure and incubation in suspension culture showed various patterns of differentiation according to the type and concentration of the test substance. In the cell groups exposed to mono, di-methylformamide, fibroblast-like differentiation was observed most frequently, while little or no epithelial differentiation was seen. These findings were in contrast to those observed in the cell groups exposed to mono, di-methylacetamide. For dimethyl sulfoxide it is considered that there is a relatively limite...Continue Reading

Citations

May 29, 2010·Orphanet Journal of Rare Diseases·Jo-David Fine

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