A study of the effects of hydrostatic pressure on macromolecular synthesis in Escherichia coli

Biophysical Journal
A A Yayanos, E C Pollard

Abstract

In cultures of Escherichia coli 15 (thymine(-), leucine(-)) which were incubated at high hydrostatic pressures, cell division occurred only at pressures below 430 atm but in a somewhat synchronous fashion at around 250 atm. The rate of leucine-(14)C incorporation into a macromolecular fraction of the cells diminished to a zero value at about 580 atm and that of uracil-(14)C incorporation to a zero value at about 770 atm. The rate of thymine-(14)C incorporation at pressures around 330 atm was that to be expected with a culture in which DNA synthesis is somewhat synchronous. At pressures above 500 atm, thymine-(14)C was incorporated only over the initial part of the pressure incubation and further incorporation under pressure was not observed no matter how long the duration of the incubation. We present evidence along several lines that the thymine incorporation kinetics reflect an effect of pressure on a locus at the origin (or termination) of a replication of the bacterial chromosome. The recovery of cell division and of the incorporation rates upon release of pressure were found to depend on the magnitude of the pressure and the duration of the pressure incubation.

Citations

Sep 2, 2004·Extremophiles : Life Under Extreme Conditions·Akihiro IshiiChiaki Kato
Jan 18, 2007·Extremophiles : Life Under Extreme Conditions·Federico M Lauro, Douglas H Bartlett
May 2, 2002·Biochimica Et Biophysica Acta·D H Bartlett
Nov 5, 1999·Trends in Microbiology·F AbeK Horikoshi
Apr 12, 2001·Journal of Applied Microbiology·A K KaratzasM H Bennik
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R B Murphy, W F Libby
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A A YayanosR Van Boxtel
Dec 13, 2006·Applied and Environmental Microbiology·Federico M LauroDouglas H Bartlett
Oct 22, 2008·Applied and Environmental Microbiology·Kai LinkeRudi F Vogel
Jun 29, 2002·Applied and Environmental Microbiology·Kimon A G Karatzas, Marjon H J Bennik
Nov 19, 2008·BMC Evolutionary Biology·Stefano CampanaroGiorgio Valle
Nov 3, 2010·Research in Microbiology·Philippe M Oger, Mohamed Jebbar
Nov 18, 2014·AMB Express·Martina SchedlerRudolf Müller
Apr 18, 2015·Journal of Food Science·Kazuki NomuraToru Shigematsu
Jun 22, 2013·Plant Physiology and Biochemistry : PPB·Wei ZhangJun Duan
Jul 9, 2013·Biotechnology Advances·Maria J MotaJorge A Saraiva
Jan 23, 2015·Frontiers in Microbiology·Angeliki MarietouDouglas H Bartlett
Jul 27, 2014·Applied and Environmental Microbiology·Angeliki Marietou, Douglas H Bartlett
Oct 13, 2019·Applied and Environmental Microbiology·Huyen Thi Minh NguyenTakashi Inaoka
May 10, 2017·Bioscience, Biotechnology, and Biochemistry·Takashi InaokaKazutaka Yamamoto
Nov 18, 2018·Folia Microbiologica·Lisa-Maria MauerhoferSimon K-M R Rittmann
Feb 1, 1975·Biophysical Chemistry·G SchmidR Jaenicke
Nov 1, 1993·Journal of Bacteriology·T J WelchD H Bartlett
Apr 1, 1986·Applied and Environmental Microbiology·E F Delong, A A Yayanos
Oct 1, 1975·Applied Microbiology·J R Schwarz, R R Colwell
Feb 1, 1971·Journal of Bacteriology·R E MarquisW O Fenn
Feb 1, 1972·Journal of Bacteriology·J R Schwarz, J V Landau

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