A study of the glycoantigens of neonatal porcine islet-like cell clusters using a lectin microarray

Transplantation Proceedings
Shino NakatsuShuji Miyagawa

Abstract

The pig pancreas is considered to be the most suitable source of islets for clinical xenotransplantation. Two types of islet transplantation are: adult pig islets and neonatal porcine islet-like cell clusters (NPCC). However, besides a-Gal expression, differences in glycosylation and xenoantigenicity between both types were not clear so fat to date. In this study, we performed lectin microarray analyses of NPCCs cultured for 1, 5, or 9 days. We studied differences in gycoantigens among several kinds of wild-type NPCCs isolated from 1- to 3-day-old neonatal wild-type pigs (Large White/Landrace × Duroc) and cultured for 1, 5 and 9 days in Ham's 10 in the presence of nicotinamide, using a previously published technique. After sonication and centrifugation, supernatant proteins from each islet were labeled with Cy3, applied to a lectin array and scanned with an SC-Profiler for evaluation using an Array Pro Analyzer. The overall signals of NPCC at days 5 and 9, showed almost the same values to most lectins, whereas those on day 1 showed differences, suggesting that the NPCC on day 1 contain immature cells that gradually turn to mature NPCCs in culture.

References

May 1, 1996·The Journal of Clinical Investigation·G S KorbuttR V Rajotte
Apr 22, 2004·Xenotransplantation·Hiroshi KomodaRyota Shirakura
Feb 13, 2010·Xenotransplantation·Shuji MiyagawaMasahiro Fukuzawa
Mar 2, 2011·Current Opinion in Organ Transplantation·Robert B Elliott, UNKNOWN Living Cell Technologies

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Citations

Aug 19, 2014·Xenotransplantation·Santosh NagarajuDavid K C Cooper
Oct 16, 2013·Current Opinion in Organ Transplantation·Santosh NagarajuDavid K C Cooper
Jan 24, 2020·Expert Review of Proteomics·Hanjie YuZheng Li

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