A study of the immune properties of human umbilical cord lining epithelial cells

Cytotherapy
Yi-Jun CaiAndrew Burd

Abstract

Our previous study has demonstrated the stem cell-like properties of human umbilical cord lining epithelial cells (CLECs) and their capability for epidermal reconstitution in organotypic skin culture; however, the immunogenicity of these cells has not been clearly defined. We assessed several aspects of the immune properties of CLECs in vitro. We examined CLECs for their immunoregulatory function in a mixed lymphocyte culture experiment. We characterized the expression patterns of the major histocompatibility complex (MHC), co-stimulatory molecules and the pro-/anti-inflammatory cytokines and growth factors in CLECs by means of reverse transcription-polymerase chain reaction, Western blotting, flow cytometry and FlowCytomix multiple analyte detection assays. CLECs were found not to induce but to suppress the proliferation response of the peripheral blood mononuclear cells in a mixed lymphocyte culture assay. They did not express the MHC class II antigen HLA-DR but the non-classic MHC class I antigens HLA-G and HLA-E and lacked the expression of the co-stimulatory molecules CD40, CD80 and CD86. In addition, they produced less interleukin-1β and transforming growth factor-β1 but more interleukin-4 and hepatocyte growth factor tha...Continue Reading

References

Sep 1, 1990·European Journal of Immunology·V BalR Lechler
May 1, 1987·Transplantation·W R GilksP T Klouda
Mar 1, 1995·Research in Immunology·C Somoza, L L Lanier
Nov 22, 1997·Current Opinion in Immunology·C P Larsen, T C Pearson
Oct 13, 2000·American Journal of Respiratory and Critical Care Medicine·L Bugeon, M J Dallman
Jan 4, 2003·Nature Reviews. Immunology·Siamon Gordon
Dec 25, 2003·The Journal of Pathology·Frank B NiessenWim Timens
Aug 6, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mark W J Ferguson, Sharon O'Kane
May 3, 2005·Journal of Biomedical Science·Elena KlyushnenkovaKevin R McIntosh
Jan 25, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Andrew BurdLin Huang
Mar 13, 2008·Blood·Edgardo D CarosellaJoel Lemaoult
Jun 25, 2008·The Annals of Thoracic Surgery·Rony AtouiRay C J Chiu
Aug 16, 2008·Stem Cells·Mark L WeissKevin R McIntosh
May 14, 2009·Transplantation·Zohair SelmaniFrédéric Deschaseaux
Jun 15, 2011·Immunotherapy·Gilles BenichouGeorges Tocco

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