PMID: 9159194Aug 1, 1996Paper

A study of the interactions of an immunoglobulin light chain with artificial and B-lymphocyte membranes

Frontiers in Bioscience : a Journal and Virtual Library
J S WallP S O'Shea

Abstract

The binding of an immunoglobulin lambda light chain (IgLC) to synthetic and biological membranes was monitored in real-time using a recently developed, time-resolved fluorescence technique. Lambda IgLC purified from the urine of patients with multiple myeloma, were used in studies of protein-membrane interactions. The association of the lambda IgLC dimer with B-lymphocytes was shown to be stabilised predominantly by non-polar interactions. Furthermore, it was found that following binding to synthetic phospholipid membranes, a reorientation of the light chain occurred which resulted in a change in the distribution of charged residues at the lipid-water interface. The rate constants associated with the binding event were calculated, and appear to comprise both temperature insensitive and sensitive components. The calculated activation energies of the binding and reorientation events were found to be 13.53 KJmol(-1) and 87.89 KJmol(-1), respectively. The large activation energy associated with the reorientation phase suggests the movement of large protein domains, possibly involving a whole immunoglobulin domain. The binding and reorganisation of the IgLC upon the phospholipid membrane may confer novel biological functions to the ...Continue Reading

Citations

Feb 7, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Esther Ganelin-CohenBatia Kaplan
Jul 17, 2003·European Journal of Clinical Investigation·G CohenW H Hörl
Aug 28, 2009·Seminars in Dialysis·Gerald Cohen, Walter H Hörl
Sep 8, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew T HutchinsonRobert L Raison
Oct 3, 2002·The Journal of Biological Chemistry·Min ZhuAnthony L Fink
Dec 7, 2006·The American Journal of Pathology·Grace Fortes MonisVickery Trinkaus-Randall
Apr 16, 2003·Kidney International. Supplement·Gerald Cohen
Jul 22, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Jordan S LanePaul O'Shea
Jul 10, 2019·Biochemistry·Pinaki MisraMarina Ramirez-Alvarado
Mar 15, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Takanari NakanoTakayuki Murakoshi

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